For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
The Abcam Dylight ® 405 Fast Conjugation Kit (ab201798) provides an easy-to-use, one-step procedure that allows researchers to covalently label proteins, peptides and other biomolecules containing primary amines with Dylight ® 405 with only 30 seconds hands-on time. Furthermore, conjugates are ready to use in less than twenty minutes.
Dylight ® 405 is a blue fluorescent dye commonly used in fluorescence microscopy, arrays and in situ hybridiszation. It has an excitation wavelength of 400 nm and an emission wavelength of 420 nm (extinction coefficient 3.0 x 104 cm-1 M-1) and high quantum yield.
Learn more about buffer compatibility, protein/secondary antibody conjugation and labeling chemistry in our FAQs.
The kit comes in 3 sizes for labelling recommended amounts of antibody:
30 μg containing 3 reactions (each for 10 - 20 μg)
300 μg containing 3 reactions (each for 100 - 200 μg)
1 mg containing 1 reaction (for 1 - 2 mg)
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
The antibody to be labelled should be purified, in an appropriate buffer for conjugation and at a suitable concentration, as described in the protocol booklet. If not, consider using our antibody purification and concentration kits.
|Components||30 µg||300 µg||1 mg|
|Dylight® 405 Conjugation Mix||3 vials||3 vials||1 vial|
|Dylight® 405 Modifier Reagent||1 vial||1 vial||1 vial|
|Dylight® 405 Quencher Reagent||1 vial||1 vial||1 vial|
Our Abpromise guarantee covers the use of ab201798 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Conjugation||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"