Overview

  • Product name
    DyLight® 405 Fast Conjugation Kit
  • Product overview

    The Abcam Dylight ® 405 Fast Conjugation Kit (ab201798) provides an easy-to-use, one-step procedure that allows researchers to covalently label proteins, peptides and other biomolecules containing primary amines with Dylight ® 405 with only 30 seconds hands-on time. Furthermore, conjugates are ready to use in less than twenty minutes. 

    Dylight ® 405 is a blue fluorescent dye commonly used in fluorescence microscopy, arrays and in situ hybridiszation. It has an excitation wavelength of 400 nm and an emission wavelength of 420 nm (extinction coefficient 3.0 x 104 cm-1 M-1) and high quantum yield.

    Learn more about buffer compatibility, protein/secondary antibody conjugation and labeling chemistry in our FAQs.

     

     

  • Notes

    The kit comes in 3 sizes for labelling recommended amounts of antibody:

    30 μg containing 3 reactions (each for 10 - 20 μg)

    300 μg containing 3 reactions (each for 100 - 200 μg)

    1 mg containing 1 reaction (for 1 - 2 mg)

    DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

     

    The antibody to be labelled should be purified, in an appropriate buffer for conjugation and at a suitable concentration, as described in the protocol booklet. If not, consider using our antibody purification and concentration kits.

  • Tested applications
    Suitable for: Conjugationmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 30 µg 300 µg 1 mg
    Dylight® 405 Conjugation Mix 3 vials 3 vials 1 vial
    Dylight® 405 Modifier Reagent 1 vial 1 vial 1 vial
    Dylight® 405 Quencher Reagent 1 vial 1 vial 1 vial
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab201798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent concentration.

Images

Protocols

References

This product has been referenced in:
  • Rizvi AH  et al. Single-cell topological RNA-seq analysis reveals insights into cellular differentiation and development. Nat Biotechnol 35:551-560 (2017). Read more (PubMed: 28459448) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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