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Dylight 488 Fast Conjugation Kit was used to label 10 µg of rat anti-TRA98 (ab82527) using Abcam's instructions.
Postnatal day 6 mouse testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% BSA in 0.1% Triton X-100 + PBS. Sections were incubated with labeled antibody diluted to 1:25 and Alexa Fluor 555 Phalloidin at 1:500 in 3% BSA in 0.1% Triton X-100 + PBS for 1 h at RT. Sections were then washed 3X with 0.1% Triton X-100 in PBS and mounted.
TRA98 is shown in green. Phalloidin, which stains F-actin is shown in red.

Mr. Bryan Niedenberger

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Submitted Jun 28 2016

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