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RecombinantRabMAb

Recombinant DyLight® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab153686)

  • Datasheet
Q&A (1)References (1)

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Abpromise

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DyLight<sup>&reg;</sup> 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab153686)

    Overview

    • Product name

      DyLight® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
      See all Rabbit isotype controls
    • Description

      DyLight® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
    • Conjugation

      DyLight® 488. Ex: 493nm, Em: 518nm
    • Tested applications

      Suitable for: ICC/IF, Flow Cytmore details
    • Immunogen

      Chemical/ Small Molecule corresponding to Human Rabbit (C terminal) conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods.KLH forms a large complex composed of ~50 kDa subunits.

    • General notes

      KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free production
      For more information see here.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Do Not Freeze.
    • Storage buffer

      pH: 7.20
      Preservative: 0.02% Sodium azide
      Constituents: 0.05% BSA, PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      EPR25A
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Growth Cone
      • Neuroscience
      • Cell Adhesion Proteins
      • ECM Proteins
      • Neuroscience
      • Neurology process
      • Neural Signal Transduction
      • Stem Cells
      • Hematopoietic Progenitors
      • Hematopoietic Stem Cells
      • Human Lineage Negative
      • Isotype/Loading Controls
      • Isotype Controls
      • Rabbit
    • Alternative names

      • rabbit isotype control

    Associated products

    • Alternative Versions

      • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
      • Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)
      • Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093)
      • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376)
      • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)
      • Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208150)
      • Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)
      • Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)
      • PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
      • Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209613)
      • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
      • PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab222107)
      • FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab223339)
      • APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)
    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    Applications

    Our Abpromise guarantee covers the use of ab153686 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF 1/50.
    Flow Cyt 1/100.

    Images

    • DyLight<sup>&reg;</sup> 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab153686)
      DyLight® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab153686)

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab153686? Please let us know so that we can cite the reference in this datasheet.

    ab153686 has been referenced in 1 publication.

    • Yodmuang S  et al. Synergistic effects of hypoxia and morphogenetic factors on early chondrogenic commitment of human embryonic stem cells in embryoid body culture. Stem Cell Rev 11:228-41 (2015). Flow Cyt . PubMed: 25618295

    Customer reviews and Q&As

    Q&A
    Submit a question

    Question

    vielen Dank für Ihre Antwort; bedauerlich, dass keine Isotop-Kontrolle zur Verfügung steht.
    Welcher "nonspecific control antibody" wird denn auf der Produktseite des Anti-NCAM antibody [EP2567Y] (DyLight® 488) genannt?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 30 2012

    Answer

    Vielen Dank für Ihre Nachfrage.

    Ich habe mich im Labor erkundigt. Als Isotype Kontrolle wurde ein Rabbit IgG Control with DyLight® 488 conjugate antibody verwendet den wir nicht im regulären Produktkatalog vertreiben.

    Ich habe mich darauf hin mit unserem Produktteam in Verbindung gesetzt. Dieser Kontrollantikörper wurde jetzt vorübergehend in unseren Katalog aufgenommen, so dass Sie diesen bestellen können wenn Sie möchten. Sie finden dieses Produkt unter:

    https://www.abcam.com/DyLightreg-488-Rabbit-IgG-monoclonal-EPR25A-Isotype-Control-ab153686.html

    Ich hoffe dies hilft Ihnen weiter. Bitte lassen Sie mich wissen wenn Sie weitere Fragen haben und ich werde gerne versuchen Ihnen zu helfen. Ich wünsche Ihnen ein schönes Wochenende.

    Read More

    Abcam Scientific Support

    Answered on Nov 30 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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