Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP801Y] to Dynamin 1 (HRP)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Conjugation: HRP
Product nameAnti-Dynamin 1 antibody [EP801Y] (HRP)
See all Dynamin 1 primary antibodies
DescriptionRabbit monoclonal [EP801Y] to Dynamin 1 (HRP)
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide within Human Dynamin 1 aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q05193
- WB: NIH3T3 whole cell lysate. IHC-P: normal human cerebral cortex tissue sections
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab207511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 97 kDa).|
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMicrotubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis.
Sequence similaritiesBelongs to the dynamin family.
Contains 1 GED domain.
Contains 1 PH domain.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Microtubule-associated.
- Information by UniProt
- B dynamin antibody
- D100 antibody
- DNM 1 antibody
IHC image of Dynamin 1 staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab207511, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-Dynamin 1 antibody [EP801Y] (HRP) (ab207511) at 1/1000 dilution + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab207511 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab207511 has not yet been referenced specifically in any publications.