Overview

  • Product name

  • Description

    Rabbit polyclonal to Dynamin 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Xenopus laevis, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Dynamin 2 (internal sequence). The exact sequence is proprietary.
    Database link: P50570

  • Positive control

    • IHC-P: Human breast carcinoma tissue. WB: PC-12, HeLa and Rat2 whole cell extracts. Rat and mouse brain tissue extracts. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/10000. Predicted molecular weight: 98 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis.
  • Tissue specificity

    Ubiquitously expressed.
  • Involvement in disease

    Defects in DNM2 are a cause of centronuclear myopathy autosomal dominant (ADCNM) [MIM:160150]; also known as autosomal dominant myotubular myopathy. Centronuclear myopathies (CNMs) are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. CNMs comprise a wide spectrum of phenotypes, ranging from severe neonatal to mild late-onset familial forms. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
    Defects in DNM2 are the cause of Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB) [MIM:606482]. Charcot-Marie-Tooth disease (CMT) is a clinically and genetically heterogeneous disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. CMTDIB is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
  • Sequence similarities

    Belongs to the dynamin family.
    Contains 1 GED domain.
    Contains 1 PH domain.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Microtubule-associated. Also found in the postsynaptic density of neuronal cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • CMT2M antibody
    • CMTDI1 antibody
    • CMTDIB antibody
    • DI CMTB antibody
    • Dnm2 antibody
    • DYN II antibody
    • DYN2 antibody
    • DYN2_HUMAN antibody
    • Dynamin II antibody
    • Dynamin-2 antibody
    • Dynamin2 antibody
    • DynaminII antibody
    • DYNII antibody
    see all

Images

  • Anti-Dynamin 2 antibody (ab227192) at 1/5000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 98 kDa



    7.5% SDS PAGE

  • Methanol fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Dynamin 2 (green) using ab227192(1/500 dilution) in ICC/IF.

    Counterstain: Hoechst 33342 (blue).

  • Paraffin embedded human breast carcinoma tissue stained for Dynamin 2 with ab227192 (1/500 dilution) in immunohistochemical analysis.

  • Anti-Dynamin 2 antibody (ab227192) at 1/5000 dilution + Mouse brain tissue extracts at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 98 kDa



    7.5% SDS-PAGE

  • Anti-Dynamin 2 antibody (ab227192) at 1/5000 dilution + Rat brain tissue extract at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 98 kDa



    7.5% SDS-PAGE

  • All lanes : Anti-Dynamin 2 antibody (ab227192) at 1/1000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extracts
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 98 kDa



    7.5% SDS-PAGE

References

ab227192 has not yet been referenced specifically in any publications.

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