Overview

  • Product name

    Anti-Dynamin 2 antibody [EPR9053(2)(ABC)]
    See all Dynamin 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR9053(2)(ABC)] to Dynamin 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Dynamin 2 aa 750-850. The exact sequence is proprietary.

  • Positive control

    • HeLa, K562, Jurkat and fetal brain lysates; Human kidney and skeletal muscle tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 98 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis.
    • Tissue specificity

      Ubiquitously expressed.
    • Involvement in disease

      Defects in DNM2 are a cause of centronuclear myopathy autosomal dominant (ADCNM) [MIM:160150]; also known as autosomal dominant myotubular myopathy. Centronuclear myopathies (CNMs) are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. CNMs comprise a wide spectrum of phenotypes, ranging from severe neonatal to mild late-onset familial forms. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
      Defects in DNM2 are the cause of Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB) [MIM:606482]. Charcot-Marie-Tooth disease (CMT) is a clinically and genetically heterogeneous disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. CMTDIB is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
    • Sequence similarities

      Belongs to the dynamin family.
      Contains 1 GED domain.
      Contains 1 PH domain.
    • Cellular localization

      Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Microtubule-associated. Also found in the postsynaptic density of neuronal cells.
    • Information by UniProt
    • Database links

    • Alternative names

      • CMT2M antibody
      • CMTDI1 antibody
      • CMTDIB antibody
      • DI CMTB antibody
      • Dnm2 antibody
      • DYN II antibody
      • DYN2 antibody
      • DYN2_HUMAN antibody
      • Dynamin II antibody
      • Dynamin-2 antibody
      • Dynamin2 antibody
      • DynaminII antibody
      • DYNII antibody
      see all

    Images

    • All lanes : Anti-Dynamin 2 antibody [EPR9053(2)(ABC)] (ab151555) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : Fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 98 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Dynamin 2 with ab151555 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labelling Dynamin 2 with ab151555 at 1/50 dilution.

    References

    This product has been referenced in:

    • Hofhuis J  et al. Dysferlin mediates membrane tubulation and links T-tubule biogenesis to muscular dystrophy. J Cell Sci 130:841-852 (2017). Read more (PubMed: 28104817) »
    See 1 Publication for this product

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