• Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    The dynamins are a family of 100 kDa GTPases transcribed from at least three separate genes. At least four mRNA splice variants for each dynamin have been described. Dynamins contain several conserved regions including the conserved, amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a coiled-coil region located in front of a proline-rich domain (PRD). The PRD is thought to mediate interactions between dynamin and numerous other cellular proteins. Dynamin 1 is expressed exclusively in neurons, Dynamin 2 is ubiquitously expressed, and Dynamin 3 is thought to be restricted to expression in the brain, testis, heart, and lung. The dynamins participate in the cellular process of clathrin-mediated and fluid-phase endocytosis.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab3458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Can be blocked with Dynamin 3 peptide (ab4986). By Western blot, this antibody detects an ~100 kDa protein representing Dynamin 3 from HeLa cell lysate.
IHC-P Use a concentration of 4 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.


  • Function
    Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis.
  • Sequence similarities
    Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.
    Contains 1 dynamin-type G (guanine nucleotide-binding) domain.
    Contains 1 GED domain.
    Contains 1 PH domain.
  • Cellular localization
    Cytoplasm. Cytoplasm, cytoskeleton. Microtubule-associated.
  • Information by UniProt
  • Database links
  • Alternative names
    • testicular dynamin antibody
    • testicular antibody
    • DNM 3 antibody
    • DNM3 antibody
    • DNM3 protein antibody
    • Dyn3 antibody
    • DYN3_HUMAN antibody
    • Dyna III antibody
    • DynaIII antibody
    • dynamin 2 antibody
    • Dynamin antibody
    • Dynamin family member antibody
    • Dynamin testicular antibody
    • Dynamin-3 antibody
    • Dynamin3 antibody
    • EC antibody
    • KIAA0820 antibody
    • MGC70433 antibody
    • T dynamin antibody
    • T-dynamin antibody
    see all


  • Western blot of Dynamin 3 on rat liver extract using ab3458.

  • ab3458 (4µg/ml) staining Dynamin 3 in human brain cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear/cytoplasmic compartments within the white matter region
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab3458 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3458, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Ding W  et al. Osteopontin deficiency ameliorates Alport pathology by preventing tubular metabolic deficits. JCI Insight 3:N/A (2018). Read more (PubMed: 29563333) »
  • Gu C  et al. Dynamin 3 suppresses growth and induces apoptosis of hepatocellular carcinoma cells by activating inducible nitric oxide synthase production. Oncol Lett 13:4776-4784 (2017). Read more (PubMed: 28599479) »
See all 8 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

1-2 of 2 Abreviews

Immunocytochemistry/ Immunofluorescence
Rat Cell (testis tissue)
testis tissue
Yes - cold acetone
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C

Dr. Wayne Vogl

Verified customer

Submitted Jan 29 2010

Western blot
Human Cell lysate - whole cell (HEK-293T)
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 13 2007

For licensing inquiries, please contact partnerships@abcam.com

Sign up