Immunocytochemistry/ Immunofluorescence abreview for Anti-Dynamin 3 antibody

Poor
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (testis tissue)
Specification
testis tissue
Fixative
Paraformaldehyde
Permeabilization
Yes - cold acetone
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Dilution
1/100
Incubation time
9 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
Used antibody to stain tubulobulbar complexes on 3% paraformaldehyde fixed and fragmented wild-type rat testis tissue. Used at a dilution of 1:100. An Alexa 488 secondary was used for detection. The expected result was staining that outlined individual complexes that are adjacent to the concave surface of late spermatid heads. No staining was observed in this region, but non-specific background staining was present.
Abcam response
Thank you for providing such valuable feedback for this antibody in the untested application, ICC/IF.

Dr. Wayne Vogl

Verified customer

Submitted Jan 29 2010

Sign up