Dynamin Inhibitors Toolbox (ab120468)

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Overview

  • Product name

    Dynamin Inhibitors Toolbox

  • Specificity

    Convenient kit of dynamin inhibitors from the MiTMAB™ and Dynole™ chemical series. Used for characterization of dynamin and endocytosis. The inhibitors have different mechanisms of action, target different domains with varying potencies, and are based on differing chemical scaffolds. Inactive controls for each series are included. Toolbox contains 1mg of each of the following: MiTMAB™ (ab120466), OcTMAB™ (ab120467), Pro-Mystyric Acid (ab120476), Dynole-34-2™ (ab120463) and Dynole-31-2™ (ab120464).

  • Product overview

    The Dynamin Inhibitors Toolbox (ab120468) is a unique collection of small molecule, potent, and cell permeable dynamin inhibitors, enabling characterization of dynamin and endocytosis. The inhibitors have different mechanisms of action, target different domains of dynamin, and are based on differing chemical scaffolds.


    Negative controls for each chemical series are included.


    The Toolbox contains 1mg of each of the following: MiTMAB™ (ab120466), OcTMAB™ (ab120467), Pro-Mystyric Acid (ab120476), Dynole-34-2™ (ab120463) and Dynole-31-2™ (ab120464).


    MiTMABTM chemical series collection 


    Contains MiTMABTM (ab120466), OcTMABTM (ab120467) and Pro-Myristic Acid (ab120476). OcTMABTM and MiTMABTM target dynamin at the lipid binding (PH) domain and inhibit dynamin and endocytosis in enzymatic and cell based assays. They are based on the same chemical scaffold.


    Pro-Myristic acid is an in vitro inhibitor of dynamin. Although cell permeable, it is rapidly broken down by cellular esterases to release intracellular myristic acid, which is not a dynamin inhibitor. It can therefore be used as a negative control in cell-based studies. 


    DynoleTM chemical series collection


    Contains dynamin inhibitor Dynole-34-2TM (ab120463) and negative control Dynole-31TM (ab120464) from the Dynole chemical series. Dynole-34-2TM targets dynamin at the GTPase Allosteric Site (GAS) domain and inhibits dynamin and endocytosis in enzymatic and cell based assays. The inhibitor and negative control are based on the same chemical scaffold. 


     


    PH lipid binding site


    MiTMAB™ - Cell-permeable dynamin I and dynamin II inhibitor 


    PH lipid binding site


    OcTMAB™ - Cell permeable dynamin I and dynamin II inhibitor 


    PH lipid binding site


    Pro-Myristic Acid - Negative control for MiTMAB™ and OcTMAB™ 


    GTPase Allosteric Site


    Dynole-34-2™ - Potent dynamin I and dynamin II inhibitor 


    GTPase Allosteric Site


    Dynole-31-2™ - Negative control for Dynole 34-2™ 

  • Notes

    Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months.

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Dynole-34-2™, Dynole-31-2™, MiTMAB™ and OcTMAB™ are trademarks of Children's Medical Research Institute and Newcastle Innovation Ltd.

  • Tested applications

    Suitable for: Functional Studiesmore details

Applications

Our Abpromise guarantee covers the use of ab120468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Functional Studies - Dynole-31-2™ (ab120464)
    Functional Studies - Dynole-31-2™ (ab120464)
    ab66705 staining PAI1 in HeLa cells treated with dynole-31-2™ (ab120464), by ICC/IF. No change in PAI1 expression with increased concentration of dynole-31-2™ (negative control for dynole 34-2™ (ab120463), as described in literature.
    The cells were incubated at 37°C for 6h in media containing different concentrations of ab120464 (dynole-31-2™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • Functional Studies - OcTMAB™ (ab120467)
    Functional Studies - OcTMAB™ (ab120467)
    ab66705 staining PAI1 in HeLa cells treated with OcTMAB™ (ab120467), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of OcTMAB™, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120467 (OcTMAB™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • Functional Studies - Dynole-34-2™ (ab120463)
    Functional Studies - Dynole-34-2™ (ab120463)

    ab66705 staining PAI1 in HeLa cells treated with dynole-34-2™; (ab120463), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of dynole-34-2™, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120463 (dynole-34-2™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

  • Functional Studies - MiTMAB™ (ab120466)
    Functional Studies - MiTMAB™ (ab120466)
    ab66705 staining PAI1 in HeLa cells treated with MiTMAB™ (ab120466), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of MiTMAB™, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120466 (MiTMAB™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab120468? Please let us know so that we can cite the reference in this datasheet.

ab120468 has been referenced in 4 publications.

  • Poudel KR  et al. Competition between TIAM1 and Membranes Balances Endophilin A3 Activity in Cancer Metastasis. Dev Cell 45:738-752.e6 (2018). PubMed: 29920278
  • Joshi S  et al. The dynamin inhibitors MiTMAB and OcTMAB induce cytokinesis failure and inhibit cell proliferation in human cancer cells. Mol Cancer Ther 9:1995-2006 (2010). PubMed: 20571068
  • Hill TA  et al. Iminochromene inhibitors of dynamins I and II GTPase activity and endocytosis. J Med Chem 53:4094-102 (2010). PubMed: 20426422
  • Hill TA  et al. Inhibition of dynamin mediated endocytosis by the dynoles--synthesis and functional activity of a family of indoles. J Med Chem 52:3762-73 (2009). PubMed: 19459681

Customer reviews and Q&As

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Question

Inquiry: I would like to know if we could obtaine samples of your Iminody22 and Iminody17 dynamin inhibitors. ab120475 and ab120468. Let me know thanks.

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Answer

Thank you for contacting us with your question.

I apologize but we only have full sizes available for each of our products, so we are unable to send small samples. We do, however, fully guarantee each of these inhibitors to meet the specifications on the corresponding datasheets.

I hope this information will be useful, but please let me know if you have any further questions or if there is anything else we might be able to do for you, and I'll be happy to help.

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