Overview

  • Product name

  • Description

    Rabbit polyclonal to DYNC1H1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human DYNC1H1 aa 1250-1300. The exact sequence is proprietary. NP_001367.2
    Database link: Q14204

  • Positive control

    • WB: HeLa, HEK-239T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245554 was affinity purified using an epitope specific to DYNC1H1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab245554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 532 kDa.

Target

  • Relevance

    Dyneins are a group of microtubule-activated ATPases that function as molecular motors. They are divided into two subgroups of axonemal and cytoplasmic dyneins. The cytoplasmic dyneins function in intracellular motility, including retrograde axonal transport, protein sorting, organelle movement, and spindle dynamics. Molecules of conventional cytoplasmic dynein are comprised of 2 heavy chain polypeptides and a number of intermediate and light chains. DYNC1H1 is a member of the cytoplasmic dynein heavy chain family which serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits.
  • Cellular localization

    Cytoplasmic, Golgi Apparatus, Microtubule.
  • Database links

  • Alternative names

    • cytoplasmic dynein 1 heavy chain 1 antibody
    • cytoplasmic dynein heavy chain 1 antibody
    • DHC1 antibody
    • DHC1a antibody
    • DKFZp686P2245 antibody
    • DNCH1 antibody
    • Dnchc1 antibody
    • DNECL antibody
    • DYHC antibody
    • dynein heavy chain, cytosolic antibody
    • dynein, cytoplasmic 1, heavy chain 1 antibody
    • dynein, cytoplasmic-like antibody
    • HL-3 antibody
    • KIAA0325 antibody
    • p22 antibody
    see all

Images

  • All lanes : Anti-DYNC1H1 antibody (ab245554) at 0.1 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 532 kDa


    Exposure time: 3 seconds


    Prepared using NETN lysis buffer.

  • DYNC1H1 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
    ab245554 used for IP at 6 µg per reaction. For WB 0.4 µg/ml.

    Lane 1: ab245554 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Chemiluminescence detection: 3 seconds.

References

ab245554 has not yet been referenced specifically in any publications.

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