Anti-DYNC1H1 antibody (ab245554)
Key features and details
- Rabbit polyclonal to DYNC1H1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DYNC1H1 antibody
See all DYNC1H1 primary antibodies -
Description
Rabbit polyclonal to DYNC1H1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human DYNC1H1 aa 1250-1300. The exact sequence is proprietary. NP_001367.2
Database link: Q14204 -
Positive control
- WB: HeLa, HEK-239T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: HEK-293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab245554 was affinity purified using an epitope specific to DYNC1H1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 532 kDa.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 532 kDa. |
Target
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Relevance
Dyneins are a group of microtubule-activated ATPases that function as molecular motors. They are divided into two subgroups of axonemal and cytoplasmic dyneins. The cytoplasmic dyneins function in intracellular motility, including retrograde axonal transport, protein sorting, organelle movement, and spindle dynamics. Molecules of conventional cytoplasmic dynein are comprised of 2 heavy chain polypeptides and a number of intermediate and light chains. DYNC1H1 is a member of the cytoplasmic dynein heavy chain family which serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. -
Cellular localization
Cytoplasmic, Golgi Apparatus, Microtubule. -
Database links
- Entrez Gene: 1778 Human
- Entrez Gene: 13424 Mouse
- Entrez Gene: 29489 Rat
- Omim: 600112 Human
- SwissProt: Q14204 Human
- SwissProt: Q9JHU4 Mouse
- SwissProt: P38650 Rat
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Alternative names
- cytoplasmic dynein 1 heavy chain 1 antibody
- cytoplasmic dynein heavy chain 1 antibody
- DHC1 antibody
see all
Images
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All lanes : Anti-DYNC1H1 antibody (ab245554) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 532 kDa
Exposure time: 3 secondsPrepared using NETN lysis buffer.
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DYNC1H1 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245554 used for IP at 6 µg per reaction. For WB 0.4 µg/ml.Lane 1: ab245554 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Chemiluminescence detection: 3 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab245554 has not yet been referenced specifically in any publications.