Overview

  • Product name

  • Description

    Rabbit polyclonal to DYNC1I2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Orangutan
  • Immunogen

    Recombinant fragment corresponding to Human DYNC1I2 aa 174-210.
    Sequence:

    EDDDVVAPKPPIEPEEEKTLKKDEENDSKAPPHELTE


    Database link: Q13409

  • Positive control

    • Human kidney tissue; RT4 and U251 MG cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab204330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500. Predicted molecular weight: 71 kDa.

Target

  • Function

    Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes.
  • Sequence similarities

    Belongs to the dynein intermediate chain family.
    Contains 7 WD repeats.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR. The phosphorylation status of Ser-90 appears to be involved in dynactin-dependent target binding.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cytoplasmic dynein 1 intermediate chain 2 antibody
    • Cytoplasmic dynein intermediate chain 2 antibody
    • cytosolic antibody
    • DC1I2_HUMAN antibody
    • DH IC 2 antibody
    • DH IC-2 antibody
    • DNCI2 antibody
    • DNCIC2 antibody
    • Dync1i2 antibody
    • Dynein intermediate chain 2 antibody
    • Dynein intermediate chain 2, cytosolic antibody
    • Dynein, cytoplasmic, intermediate polypeptide 2 antibody
    • IC2 antibody
    see all

Images

  • All lanes : Anti-DYNC1I2 antibody (ab204330) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U251 MG cell lysate

    Developed using the ECL technique.

    Predicted band size: 71 kDa

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DYNC1I2 with ab204330 at 1/500 dilution.

References

ab204330 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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