Key features and details
- Rabbit polyclonal to DYNC1I2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DYNC1I2 antibody
See all DYNC1I2 primary antibodies
DescriptionRabbit polyclonal to DYNC1I2
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan
- IHC-P: Human kidney tissue; WB: RT4 and U-251 MG cell lysates. ICC/IF: U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab204330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 71 kDa.|
FunctionActs as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes.
Sequence similaritiesBelongs to the dynein intermediate chain family.
Contains 7 WD repeats.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. The phosphorylation status of Ser-90 appears to be involved in dynactin-dependent target binding.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Cytoplasmic dynein 1 intermediate chain 2 antibody
- Cytoplasmic dynein intermediate chain 2 antibody
- cytosolic antibody
All lanes : Anti-DYNC1I2 antibody (ab204330) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Developed using the ECL technique.
Predicted band size: 71 kDa
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DYNC1I2 with ab204330 at 1/500 dilution.
U-2 OS cells stained for DYNC1I2 (green) using ab204330 at 2 µg/ml in ICC/IF.
ab204330 has not yet been referenced specifically in any publications.