Key features and details
- Rabbit polyclonal to DYNC2H1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DYNC2H1 antibody
See all DYNC2H1 primary antibodies
DescriptionRabbit polyclonal to DYNC2H1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- WB: Recombinant Human DYNC2H1 protein with N-terminal GST tag. IHC-P: Human testis and small intestine tissues. ICC/IF: HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab225946 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 493 kDa.|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionMay function as a motor for intraflagellar retrograde transport. Functions in cilia biogenesis. May play a role in transport between endoplasmic reticulum and Golgi or organization of the Golgi in cells.
Involvement in diseaseDefects in DYNC2H1 are the cause of asphyxiating thoracic dystrophy type 3 (ATD3) [MIM:613091]. ATD3 is an autosomal recessive osteochondrodysplasia which often leads to death in infancy because of a severely constricted thoracic cage and respiratory insufficiency.
Defects in DYNC2H1 are the cause of short rib-polydactyly syndrome type 3 (SRPS3) [MIM:263510]; also called Verma-Naumoff syndrome. A lethal skeletal dysplasia characterized by markedly short ribs, short limbs, polydactyly, narrow thorax, and multiple anomalies of major organs, including heart, intestines, genitalia, kidney, liver, and pancreas.
Sequence similaritiesBelongs to the dynein heavy chain family.
Cellular localizationCytoplasm > cytoskeleton > cilium axoneme. Cell membrane. Cytoplasm. Localizes to the apical cytoplasm (By similarity). According to PubMed:8666668, it localizes to Golgi apparatus, cytoplasmic vesicle and endoplasmic reticulum.
- Information by UniProt
- Cytoplasmic dynein 2 heavy chain 1 antibody
- Cytoplasmic dynein 2 heavy chain antibody
- DYHC2_HUMAN antibody
All lanes : Anti-DYNC2H1 antibody (ab225946) at 1/500 dilution
Lane 1 : Recombinant Human DYNC2H1 protein with N-terminal GST tag, 80 ng
Lane 2 : Recombinant Human DYNC2H1 protein with N-terminal GST tag, 40 ng
Lane 3 : Recombinant Human DYNC2H1 protein with N-terminal GST tag, 20 ng
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 493 kDa
Predicted MW of recombinant protein: 45 kDa.
Paraffin-embedded human testis tissue stained for DYNC2H1 with ab225946 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human small intestine tissue stained for DYNC2H1 with ab225946 at 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for DYNC2H1 (green) using ab225946 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225946 has not yet been referenced specifically in any publications.