Key features and details
- Rabbit polyclonal to Dynein axonemal intermediate chain
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Dynein axonemal intermediate chain antibody
See all Dynein axonemal intermediate chain primary antibodies
DescriptionRabbit polyclonal to Dynein axonemal intermediate chain
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rhesus monkey
Recombinant fragment within Human Dynein axonemal intermediate chain 1 (internal sequence). The exact sequence is proprietary.
Database link: O14576
- WB: Jurkat and NCI-H929 whole cell lysates. IHC-P: U87 xenograft tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 73 kDa.|
FunctionActs as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. May play a role in mediating the interaction of cytoplasmic dynein with membranous organelles and kinetochores.
Sequence similaritiesBelongs to the dynein intermediate chain family.
Contains 7 WD repeats.
Cellular localizationCytoplasm. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole.
- Information by UniProt
- Cytoplasmic dynein 1 intermediate chain 1 antibody
- Cytoplasmic dynein intermediate chain 1 antibody
- cytosolic antibody
All lanes : Anti-Dynein axonemal intermediate chain antibody (ab227146) at 1/3000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : NCI-H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 73 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded U87 xenograft tissue stained for Dynein axonemal intermediate chain 1 using ab227146 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227146 has not yet been referenced specifically in any publications.