Overview

  • Product name

    Anti-Dynein axonemal intermediate chain antibody
    See all Dynein axonemal intermediate chain primary antibodies
  • Description

    Rabbit polyclonal to Dynein axonemal intermediate chain
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Dynein axonemal intermediate chain 1 (internal sequence). The exact sequence is proprietary.
    Database link: O14576

  • Positive control

    • WB: Jurkat and NCI-H929 whole cell lysates. IHC-P: U87 xenograft tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 73 kDa.

Target

  • Function

    Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. May play a role in mediating the interaction of cytoplasmic dynein with membranous organelles and kinetochores.
  • Sequence similarities

    Belongs to the dynein intermediate chain family.
    Contains 7 WD repeats.
  • Cellular localization

    Cytoplasm. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cytoplasmic dynein 1 intermediate chain 1 antibody
    • Cytoplasmic dynein intermediate chain 1 antibody
    • cytosolic antibody
    • DC1I1_HUMAN antibody
    • DH IC 1 antibody
    • DH IC-1 antibody
    • DH IC1 antibody
    • DNCI1 antibody
    • DNCIC1 antibody
    • Dync1i1 antibody
    • Dynein cytoplasmic 1 intermediate chain 1 antibody
    • Dynein intermediate chain 1 antibody
    • Dynein intermediate chain 1, cytosolic antibody
    • Dynein, cytoplasmic, intermediate polypeptide 1 antibody
    see all

Images

  • All lanes : Anti-Dynein axonemal intermediate chain antibody (ab227146) at 1/3000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : NCI-H929 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 73 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded U87 xenograft tissue stained for Dynein axonemal intermediate chain 1 using ab227146 at 1/500 dilution in immunohistochemical analysis.

References

ab227146 has not yet been referenced specifically in any publications.

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