Overview

  • Product name

    Anti-Dynein heavy chain antibody
    See all Dynein heavy chain primary antibodies
  • Description

    Rabbit polyclonal to Dynein heavy chain
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 4206-4255 (DSQARDGAGA TREEKVKALL EEILERVTDE FNIPELMAKV EERTPYIVVA) of Human Dynein heavy chain (NP_004653)

  • Positive control

    • Human placenta tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab133968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 512 kDa.
ICC/IF 1/50.

Target

  • Function

    Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP.
  • Sequence similarities

    Belongs to the dynein heavy chain family.
  • Domain

    Dynein heavy chains probably consist of an N-terminal stem (which binds cargo and interacts with other dynein components), and the head or motor domain. The motor contains six tandemly-linked AAA domains in the head, which form a ring. A stalk-like structure (formed by two of the coiled coil domains) protrudes between AAA 4 and AAA 5 and terminates in a microtubule-binding site. A seventh domain may also contribute to this ring; it is not clear whether the N-terminus or the C-terminus forms this extra domain. There are four well-conserved and two non-conserved ATPase sites, one per AAA domain. Probably only one of these (within AAA 1) actually hydrolyzes ATP, the others may serve a regulatory function.
  • Cellular localization

    Cytoplasm, cytoskeleton, cilium axoneme.
  • Information by UniProt
  • Database links

  • Alternative names

    • axonemal antibody
    • Axonemal beta dynein heavy chain 9 antibody
    • Ciliary dynein heavy chain 9 antibody
    • Ciliary dynein heavy chain antibody
    • DNAH 9 antibody
    • DNAH17L antibody
    • DNAH9 antibody
    • DNAH9 variant protein antibody
    • Dnahc 9 antibody
    • Dnahc9 antibody
    • DNAL 1 antibody
    • DNAL1 antibody
    • DNEL 1 antibody
    • DNEL1 antibody
    • DYH 9 antibody
    • DYH9 antibody
    • DYH9_HUMAN antibody
    • Dynein axonemal heavy chain 9 antibody
    • Dynein axonemal heavy polypeptide 17 like antibody
    • Dynein axonemal heavy polypeptide 9 antibody
    • Dynein heavy chain 9 antibody
    • dynein heavy chain 9, axonemal antibody
    • HL 20 antibody
    • HL20 antibody
    • KIAA0357 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of mouse tracheal epithelial cells labelling Dynein heavy chain with ab133968 at 1/50. An Alexa Fluor 488-conjugtaed anti-rabbit IgG (1/1000) was used as the secondary antibody.

  • Immunocytochemistry/Immunofluorescence analysis of mouse tracheal epithelial cells labelling Dynein heavy chain with ab133968 at 1/50. An Alexa Fluor 488-conjugtaed anti-rabbit IgG (1/1000) was used as the secondary antibody.

  • Anti-Dynein heavy chain antibody (ab133968) at 1 µg/ml + Human placenta tissue lysate at 10 µg

    Predicted band size: 512 kDa



    The predicted MW of Isoform 3 (UniProt Q9NYC9-3) is 91 kDa.

References

This product has been referenced in:

  • Ha S  et al. Mutations in Dnaaf1 and Lrrc48 Cause Hydrocephalus, Laterality Defects, and Sinusitis in Mice. G3 (Bethesda) 6:2479-87 (2016). IHC . Read more (PubMed: 27261005) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

Based on the sequence of the immunizing peptide, there is 100% homology to isoforms 1, 2 and 3 (http://www.uniprot.org/uniprot/Q9NYC9). Unfortunately, we do not have data showing the presence of a ˜500kD band at this time.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up