Recombinant
RabMAb

Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964)

Overview

  • Product name
    Anti-Dynein intermediate chain 1 antibody [EPR11244-61]
    See all Dynein intermediate chain 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11244-61] to Dynein intermediate chain 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Axonemal dynein intermediate chain 1 aa 1-200. The exact sequence is proprietary.
    Database link: Q9UI46

  • Positive control
    • Human, rat and mouse testis lysates; Human testis and trachea tissues; Jurkat cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab171964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 79 kDa.
ICC/IF 1/50 - 1/100.

For unpurified, use 1/50 - 1/100.

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Part of the dynein complex of respiratory cilia.
    • Involvement in disease
      Defects in DNAI1 are the cause of primary ciliary dyskinesia type 1 (CILD1) [MIM:244400]. CILD1 is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome.
      Defects in DNAI1 are the cause of Kartagener syndrome (KTGS) [MIM:244400]. KTGS is an autosomal recessive disorder characterized by the association of primary ciliary dyskinesia with situs inversus. Clinical features include recurrent respiratory infections, bronchiectasis, infertility, and lateral transposition of the viscera of the thorax and abdomen. The situs inversus is most often total, although it can be partial in some cases (isolated dextrocardia or isolated transposition of abdominal viscera).
    • Sequence similarities
      Belongs to the dynein intermediate chain family.
      Contains 5 WD repeats.
    • Cellular localization
      Cytoplasm > cytoskeleton > cilium axoneme.
    • Information by UniProt
    • Database links
    • Alternative names
      • Axonemal dynein intermediate chain 1 antibody
      • Axonemal dynein intermediate chain 2 antibody
      • CILD 1 antibody
      • CILD1 antibody
      • Cytoplasmic dynein 1 intermediate chain 1 antibody
      • Cytoplasmic dynein 1 intermediate chain 2 antibody
      • Cytoplasmic dynein intermediate chain 1 antibody
      • Cytoplasmic dynein intermediate chain 2 antibody
      • DH IC 1 antibody
      • DH IC 2 antibody
      • DIC1 antibody
      • DNAI 1 antibody
      • DNAI 2 antibody
      • DNAI1 antibody
      • DNAI1_HUMAN antibody
      • DNAI2 antibody
      • DNCI 2 antibody
      • DNCI1 antibody
      • DNCI2 antibody
      • DNCIC 1 antibody
      • DNCIC 2 antibody
      • DNCIC1 antibody
      • DNCIC2 antibody
      • Dynein axonemal intermediate chain 1 antibody
      • Dynein axonemal intermediate polypeptide 1 antibody
      • Dynein axonemal intermediate polypeptide 2 antibody
      • Dynein cytoplasmic intermediate polypeptide 1 antibody
      • Dynein cytoplasmic intermediate polypeptide 2 antibody
      • Dynein intermediate chain 1 axonemal antibody
      • Dynein intermediate chain 1 cytosolic antibody
      • Dynein intermediate chain 1, axonemal antibody
      • Dynein intermediate chain 2 axonemal antibody
      • Dynein intermediate chain 2 cytosolic antibody
      • Dynein intermediate chain DNAI1 antibody
      • IC74 antibody
      • ICS antibody
      • ICS1 antibody
      • Immotile cilia syndrome 1 antibody
      • MGC26204 antibody
      • PCD antibody
      see all

    Images

    • Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964) at 1/5000 dilution (purified) + Human testis tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 79 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964) at 1/1000 dilution (purified) + Rat testis lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 79 kDa
      Observed band size: 79 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964) at 1/5000 dilution (purified) + Mouse testis tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 79 kDa
      Observed band size: 79 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunofluorescence staining of F9 cells with purified ab171964 at a working dilution of 1 in 250, counter-stained with DAPI and mouse monoclonal anti-tubulin (ab7291, 1/500). The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom right hand and middle panels - for the negative control, purified ab171964 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500.

    • All lanes : Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964) at 1/1000 dilution (unpurified)

      Lane 1 : Human testis lysate
      Lane 2 : rat testis lysate
      Lane 3 : mouse testis lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 79 kDa

    • Immunofluorescence analysis of Jurkat cells, labeling Dynein intermediate chain 1 using unpurified ab171964 at a 1/50 dilution (green) and DAPI nuclear staining (blue).

    References

    ab171964 has not yet been referenced specifically in any publications.

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