Recombinant
RabMAb

Recombinant Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] (ab156567)

Overview

  • Product name

    Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222]
    See all Dynein intermediate chain 1/DNAI1 primary antibodies
  • Description

    Rabbit monoclonal [EPR11222] to Dynein intermediate chain 1/DNAI1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human Dynein intermediate chain 1/DNAI1.
    Database link: O14576

  • Positive control

    • Fetal brain and Human hypothalamus lysates
  • General notes

     This product was previously labelled as Dynein intermediate chain 1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab156567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 79 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Part of the dynein complex of respiratory cilia.
    • Involvement in disease

      Defects in DNAI1 are the cause of primary ciliary dyskinesia type 1 (CILD1) [MIM:244400]. CILD1 is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome.
      Defects in DNAI1 are the cause of Kartagener syndrome (KTGS) [MIM:244400]. KTGS is an autosomal recessive disorder characterized by the association of primary ciliary dyskinesia with situs inversus. Clinical features include recurrent respiratory infections, bronchiectasis, infertility, and lateral transposition of the viscera of the thorax and abdomen. The situs inversus is most often total, although it can be partial in some cases (isolated dextrocardia or isolated transposition of abdominal viscera).
    • Sequence similarities

      Belongs to the dynein intermediate chain family.
      Contains 5 WD repeats.
    • Cellular localization

      Cytoplasm > cytoskeleton > cilium axoneme.
    • Information by UniProt
    • Database links

    • Alternative names

      • Axonemal dynein intermediate chain 1 antibody
      • Axonemal dynein intermediate chain 2 antibody
      • CILD 1 antibody
      • CILD1 antibody
      • Cytoplasmic dynein 1 intermediate chain 1 antibody
      • Cytoplasmic dynein 1 intermediate chain 2 antibody
      • Cytoplasmic dynein intermediate chain 1 antibody
      • Cytoplasmic dynein intermediate chain 2 antibody
      • DH IC 1 antibody
      • DH IC 2 antibody
      • DIC1 antibody
      • DNAI 1 antibody
      • DNAI 2 antibody
      • DNAI1 antibody
      • DNAI1_HUMAN antibody
      • DNAI2 antibody
      • DNCI 2 antibody
      • DNCI1 antibody
      • DNCI2 antibody
      • DNCIC 1 antibody
      • DNCIC 2 antibody
      • DNCIC1 antibody
      • DNCIC2 antibody
      • Dynein axonemal intermediate chain 1 antibody
      • Dynein axonemal intermediate polypeptide 1 antibody
      • Dynein axonemal intermediate polypeptide 2 antibody
      • Dynein cytoplasmic intermediate polypeptide 1 antibody
      • Dynein cytoplasmic intermediate polypeptide 2 antibody
      • Dynein intermediate chain 1 axonemal antibody
      • Dynein intermediate chain 1 cytosolic antibody
      • Dynein intermediate chain 1, axonemal antibody
      • Dynein intermediate chain 2 axonemal antibody
      • Dynein intermediate chain 2 cytosolic antibody
      • Dynein intermediate chain DNAI1 antibody
      • IC74 antibody
      • ICS antibody
      • ICS1 antibody
      • Immotile cilia syndrome 1 antibody
      • MGC26204 antibody
      • PCD antibody
      see all

    Images

    • All lanes : Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] (ab156567) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : Human hypothalamus lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 79 kDa
      Observed band size: 74 kDa
      why is the actual band size different from the predicted?

    • Immunoprecipitation. ab156567 at 1/1000 staining Dynein intermediate chain 1/DNAI1 in fetal brain lysate immunoprecipitated using ab156567 at 1/10. Predicted band size: 74 kDa.

    References

    ab156567 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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