Product nameAnti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222]
See all Dynein intermediate chain 1/DNAI1 primary antibodies
DescriptionRabbit monoclonal [EPR11222] to Dynein intermediate chain 1/DNAI1
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human Dynein intermediate chain 1/DNAI1.
Database link: O14576
- Fetal brain and Human hypothalamus lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab156567 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 79 kDa).|
|IP||1/10 - 1/100.|
FunctionPart of the dynein complex of respiratory cilia.
Involvement in diseaseDefects in DNAI1 are the cause of primary ciliary dyskinesia type 1 (CILD1) [MIM:244400]. CILD1 is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome.
Defects in DNAI1 are the cause of Kartagener syndrome (KTGS) [MIM:244400]. KTGS is an autosomal recessive disorder characterized by the association of primary ciliary dyskinesia with situs inversus. Clinical features include recurrent respiratory infections, bronchiectasis, infertility, and lateral transposition of the viscera of the thorax and abdomen. The situs inversus is most often total, although it can be partial in some cases (isolated dextrocardia or isolated transposition of abdominal viscera).
Sequence similaritiesBelongs to the dynein intermediate chain family.
Contains 5 WD repeats.
Cellular localizationCytoplasm > cytoskeleton > cilium axoneme.
- Information by UniProt
- Axonemal dynein intermediate chain 1 antibody
- Axonemal dynein intermediate chain 2 antibody
- CILD 1 antibody
All lanes : Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] (ab156567) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Human hypothalamus lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 79 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Immunoprecipitation. ab156567 at 1/1000 staining Dynein intermediate chain 1/DNAI1 in fetal brain lysate immunoprecipitated using ab156567 at 1/10. Predicted band size: 74 kDa.
ab156567 has not yet been referenced specifically in any publications.