Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11222] to Dynein intermediate chain 1/DNAI1 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] - BSA and Azide free
See all Dynein intermediate chain 1/DNAI1 primary antibodies
DescriptionRabbit monoclonal [EPR11222] to Dynein intermediate chain 1/DNAI1 - BSA and Azide free
Tested applicationsSuitable for: IP, WBmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human Dynein intermediate chain 1/DNAI1. The exact sequence is proprietary.
Database link: O14576
ab249262 is the carrier-free version of ab156567 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab249262 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Dynein intermediate chain 1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
Our Abpromise guarantee covers the use of ab249262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 74 kDa (predicted molecular weight: 79 kDa).|
FunctionPart of the dynein complex of respiratory cilia.
Involvement in diseaseDefects in DNAI1 are the cause of primary ciliary dyskinesia type 1 (CILD1) [MIM:244400]. CILD1 is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome.
Defects in DNAI1 are the cause of Kartagener syndrome (KTGS) [MIM:244400]. KTGS is an autosomal recessive disorder characterized by the association of primary ciliary dyskinesia with situs inversus. Clinical features include recurrent respiratory infections, bronchiectasis, infertility, and lateral transposition of the viscera of the thorax and abdomen. The situs inversus is most often total, although it can be partial in some cases (isolated dextrocardia or isolated transposition of abdominal viscera).
Sequence similaritiesBelongs to the dynein intermediate chain family.
Contains 5 WD repeats.
Cellular localizationCytoplasm > cytoskeleton > cilium axoneme.
- Information by UniProt
- Axonemal dynein intermediate chain 1 antibody
- Axonemal dynein intermediate chain 2 antibody
- CILD 1 antibody
All lanes : Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] (ab156567) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Human hypothalamus lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 79 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
This data was developed using ab156567, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab249262 has not yet been referenced specifically in any publications.