Recombinant Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] - BSA and Azide free (ab249677)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11244-61] to Dynein intermediate chain 1/DNAI1 - BSA and Azide free
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] - BSA and Azide free
See all Dynein intermediate chain 1/DNAI1 primary antibodies -
Description
Rabbit monoclonal [EPR11244-61] to Dynein intermediate chain 1/DNAI1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Dynein intermediate chain 1/DNAI1 aa 1-200. The exact sequence is proprietary.
Database link: Q9UI46 -
General notes
ab249677 is the carrier-free version of ab171964.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11244-61 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249677 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 79 kDa.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 79 kDa. |
Target
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Function
Part of the dynein complex of respiratory cilia. -
Involvement in disease
Defects in DNAI1 are the cause of primary ciliary dyskinesia type 1 (CILD1) [MIM:244400]. CILD1 is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome.
Defects in DNAI1 are the cause of Kartagener syndrome (KTGS) [MIM:244400]. KTGS is an autosomal recessive disorder characterized by the association of primary ciliary dyskinesia with situs inversus. Clinical features include recurrent respiratory infections, bronchiectasis, infertility, and lateral transposition of the viscera of the thorax and abdomen. The situs inversus is most often total, although it can be partial in some cases (isolated dextrocardia or isolated transposition of abdominal viscera). -
Sequence similarities
Belongs to the dynein intermediate chain family.
Contains 5 WD repeats. -
Cellular localization
Cytoplasm > cytoskeleton > cilium axoneme. - Information by UniProt
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Database links
- Entrez Gene: 27019 Human
- Entrez Gene: 68922 Mouse
- Entrez Gene: 500442 Rat
- Omim: 603772 Human
- SwissProt: Q9UI46 Human
- SwissProt: Q8C0M8 Mouse
- SwissProt: Q5XIL8 Rat
- Unigene: 112667 Human
see all -
Alternative names
- Axonemal dynein intermediate chain 1 antibody
- Axonemal dynein intermediate chain 2 antibody
- CILD 1 antibody
see all
Images
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Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964) at 1/5000 dilution (purified) + Human testis tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDaThis data was developed using ab171964, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964) at 1/1000 dilution (purified) + Rat testis lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDaThis data was developed using ab171964, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964) at 1/5000 dilution (purified) + Mouse testis tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDaThis data was developed using ab171964, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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This data was developed using ab171964, the same antibody clone in a different buffer formulation.Immunofluorescence staining of F9 cells with purified ab171964 at a working dilution of 1 in 250, counter-stained with DAPI and mouse monoclonal anti-tubulin (ab7291, 1/500). The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom right hand and middle panels - for the negative control, purified ab171964 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500.
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All lanes : Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964) at 1/1000 dilution (unpurified)
Lane 1 : Human testis tissue lysate
Lane 2 : Rat testis tissue lysate
Lane 3 : Mouse testis tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 79 kDaThis data was developed using ab171964, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249677 has not yet been referenced specifically in any publications.