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Anti-Dynein intermediate chain 2 antibody [EPR11224]
Rabbit monoclonal [EPR11224] to Dynein intermediate chain 2
IP more details Unsuitable for:
Mouse, Rat, Human
Recombinant fragment within Human Dynein intermediate chain 2. The exact sequence is proprietary.
Database link: Q9GZS0
Human testis, Rat testis, Mouse testis and fetal lung lysates.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
1/100 - 1/250.
1/10 - 1/100.
Is unsuitable for ICC/IF.
Part of the dynein complex of respiratory cilia.
Highly expressed in trachea and testis.
Involvement in disease
Defects in DNAI2 are the cause of primary ciliary dyskinesia type 9 (CILD9) [MIM:612444]. CILD is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome.
Belongs to the dynein intermediate chain family.
Contains 5 WD repeats.
Cytoplasm > cytoskeleton > cilium axoneme.
Information by UniProt
Axonemal dynein intermediate chain 2 antibody
Immunoprecipitation - Anti-Dynein intermediate chain 2 antibody [EPR11224] (ab170920)
Anti-Dynein intermediate chain 2 antibody [EPR11224] (ab170920) at 1/1000 dilution + Immunoprecipitation pellet from human testis lysate using ab170920 at 1/10.
Secondary Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution Developed using the ECL technique. Additional bands at: ~50 kDa (possible IgG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynein intermediate chain 2 antibody [EPR11224] (ab170920)
Immunohistochemical analysis of paraffin embedded testis tissue labeling Dynein intermediate chain 2 with ab170920 at 1/100.
Western blot - Anti-Dynein intermediate chain 2 antibody [EPR11224] (ab170920)
All lanes : Anti-Dynein intermediate chain 2 antibody [EPR11224] (ab170920) at 1/1000 dilution Lane 1 : Human testis lysate Lane 2 : Rat testis lysate Lane 3 : Mouse testis lysate Lane 4 : Fetal lung lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution Developed using the ECL technique. Predicted band size: 69 kDa
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"