Recombinant
RabMAb

Anti-Dynein intermediate chain 2 antibody [EPR11224] (ab170920)

Overview

  • Product name
    Anti-Dynein intermediate chain 2 antibody [EPR11224]
  • Description
    Rabbit monoclonal [EPR11224] to Dynein intermediate chain 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Dynein intermediate chain 2. The exact sequence is proprietary.
    Database link: Q9GZS0

  • Positive control
    • Human testis, Rat testis, Mouse testis and fetal lung lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11224
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
IHC-P 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Part of the dynein complex of respiratory cilia.
    • Tissue specificity
      Highly expressed in trachea and testis.
    • Involvement in disease
      Defects in DNAI2 are the cause of primary ciliary dyskinesia type 9 (CILD9) [MIM:612444]. CILD is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome.
    • Sequence similarities
      Belongs to the dynein intermediate chain family.
      Contains 5 WD repeats.
    • Cellular localization
      Cytoplasm > cytoskeleton > cilium axoneme.
    • Information by UniProt
    • Database links
    • Alternative names
      • axonemal antibody
      • Axonemal dynein intermediate chain 2 antibody
      • CILD9 antibody
      • DNAI 2 antibody
      • Dnai2 antibody
      • DNAI2_HUMAN antibody
      • Dynein axonemal intermediate chain 2 antibody
      • Dynein axonemal intermediate polypeptide 2 antibody
      • Dynein intermediate chain 2 antibody
      • Dynein intermediate chain 2 axonemal antibody
      see all

    Images

    • Anti-Dynein intermediate chain 2 antibody [EPR11224] (ab170920) at 1/1000 dilution + Immunoprecipitation pellet from human testis lysate using ab170920 at 1/10.

      Secondary
      Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Developed using the ECL technique.

      Additional bands at: ~50 kDa (possible IgG)

    • Immunohistochemical analysis of paraffin embedded testis tissue labeling Dynein intermediate chain 2 with ab170920 at 1/100.

    • All lanes : Anti-Dynein intermediate chain 2 antibody [EPR11224] (ab170920) at 1/1000 dilution

      Lane 1 : Human testis lysate
      Lane 2 : Rat testis lysate
      Lane 3 : Mouse testis lysate
      Lane 4 : Fetal lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 69 kDa

    References

    ab170920 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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