Anti-Dynein light chain antibody (ab236594)
Key features and details
- Rabbit polyclonal to Dynein light chain
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Dynein light chain antibody
See all Dynein light chain primary antibodies -
Description
Rabbit polyclonal to Dynein light chain -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Pig -
Immunogen
Recombinant full length protein corresponding to Human Dynein light chain aa 1 to the C-terminus.
Database link: O96015 -
Positive control
- WB: Mouse gonadal and brain lysates; HL-60 and NIH/3T3 whole cell lysates. IHC-P: Human testis and breast cancer tissues. ICC/IF: MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 12 kDa.
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IHC-P |
1/20 - 1/200.
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 12 kDa. |
IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity. -
Sequence similarities
Belongs to the dynein light chain family. -
Cellular localization
Cytoplasm > cytoskeleton > cilium axoneme. - Information by UniProt
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Database links
- Entrez Gene: 618379 Cow
- Entrez Gene: 10126 Human
- Entrez Gene: 54152 Mouse
- Entrez Gene: 100520304 Pig
- Omim: 610565 Human
- SwissProt: Q32KN5 Cow
- SwissProt: O96015 Human
- SwissProt: Q9DCM4 Mouse
see all -
Alternative names
- D15Ertd424e antibody
- Dnal4 antibody
- DNAL4_HUMAN antibody
see all
Images
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All lanes : Anti-Dynein light chain antibody (ab236594) at 1/500 dilution
Lane 1 : Mouse gonadal lysate
Lane 2 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lane 3 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lane 4 : Mouse brain lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 12 kDa -
Paraffin-embedded human testis tissue stained for Dynein light chain using ab236594 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human breast cancer tissue stained for Dynein light chain using ab236594 at 1/100 dilution in immunohistochemical analysis.
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MCF7 (human breast adenocarcinoma cell line) cells stained for Dynein light chain (green) using ab236594 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236594 has not yet been referenced specifically in any publications.