Overview

  • Product name

  • Description

    Rabbit polyclonal to Dynorphin A
  • Host species

    Rabbit
  • Specificity

    This antibody blocks with dynorphin 1-17 and 1-8 but not with other related peptides. Cells are found in the paraventricular and supraoptic nucleus and fibers are found in the lateral hypothalamus in rat brain of 4% FA perfused colchicine tissue.
  • Tested applications

    Suitable for: IHC-Fr, IHC-P, RIAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig, Rhesus monkey
    Predicted to work with: Guinea pig, Cow
  • Immunogen

    Synthetic peptide:

    YGGFLRRIRPKLKWDNQ

    (Pig).

  • General notes

    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, Whole serum, 0.1% BSA
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100 - 1/1500.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
RIA 1/10000.

Target

  • Relevance

    Dynorphins are a class of peptides produced by many different populations of neurons, which has some opiate like activity. It is thus classed as an endogenous opioid peptide. The dynorphins, which include dynorphin A, dynorphin B, alpha and beta neoendorphin, and big dynorphin, are all the products of a single gene, 'preprodynorphin'. Dynorphin is produced in many different parts of the brain, including the hypothalamus, the hippocampus and the spinal cord, and has many different physiological actions, depending upon its site of production. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. As such, it may help some individuals against addiction. Blocking dynorphin may help alleviate depression.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • ADCA antibody
    • Beta neoendorphin dynorphin antibody
    • Dyn A17 antibody
    • leu-enkephalin antibody
    • leumorphin antibody
    • neoendorphin-dynorphin-enkephalin prepropeptide antibody
    • PDYN antibody
    • PENKB antibody
    • Preprodynorphin antibody
    • Proenkephalin B antibody
    • rimorphin antibody
    • SCA23 antibody
    see all

References

This product has been referenced in:

  • Olsen J  et al. Intimate associations between the endogenous opiate systems and the growth hormone-releasing hormone system in the human hypothalamus. Neuroscience 258:238-45 (2014). Read more (PubMed: 24239719) »
  • Busceti CL  et al. Lack or inhibition of dopaminergic stimulation induces a development increase of striatal tyrosine hydroxylase-positive interneurons. PLoS One 7:e44025 (2012). IHC . Read more (PubMed: 23028485) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your call yesterday and for your question about these antibodies. I apologize for the delay getting back to you.

We have a general IHC protocol for FFPE sections that includes a section on antigen retrieval-

https://www.abcam.com/index.html?pageconfig=resource&rid=11488

However, these methods are optimized for slide-mounted tissue, so I have found some other resources that would be more useful for free floation sections-

http://www.ihcworld.com/_protocols/epitope_retrieval/free_floating_sections.htm

I've also attached a couple references for free floating antigen retrieval. You should be able to perform this step in a 24-well plate so it will be compatible with your current staining protocol.

I hope this information will be useful, but if you have any further questions or need anything else, please let me know and I'll be happy to help you further.

Read More

Answer

1. The serum is diluted in PBS, unfortunately we do not have the exact amount that is diluted, however we can estimate the specific antibody concentration to be 0.5-1.0mg/ml. 2. Unfortunately, I have been unsuccessful in locating information to suggest that the immunogen was conjugated to a carrier. 3. We do recommend heat treatment prior to immunostaining of paraffin sections and we have on our website a protocol you might find useful. In the case of Ab11134, heat treatment is essential. Good luck and don't hesitate to contact me for further information.

Read More

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