Product nameAnti-Dynorphin A antibody
See all Dynorphin A primary antibodies
DescriptionRabbit polyclonal to Dynorphin A
SpecificityThis antibody shows specificity of: Dynorphin A (1-13) 100%; [Leu5]-Enkephalin 0%; [Met5]-Enkephalin 0% and b-Endorphin (human) 0%.
Tested applicationsSuitable for: IHC-P, ELISA, RIA, ICCmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Mouse, Rat, Guinea pig, Cow, Chimpanzee, Zebrafish, Baboon, Cynomolgus monkey, Macaque monkey, Rhesus monkey, Gorilla
Storage instructionsStore at +4°C short term (1-2 weeks) (add antibacterial agent). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: Whole serum
Our Abpromise guarantee covers the use of ab40647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ELISA||Use at an assay dependent concentration. Cell culture filtrate or whole cells directly coated onto microtitre wells can be used.|
|RIA||Use at an assay dependent concentration.
With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
|ICC||Use at an assay dependent concentration.|
RelevanceDynorphins are a class of peptides produced by many different populations of neurons, which has some opiate like activity. It is thus classed as an endogenous opioid peptide. The dynorphins, which include dynorphin A, dynorphin B, alpha and beta neoendorphin, and big dynorphin, are all the products of a single gene, 'preprodynorphin'. Dynorphin is produced in many different parts of the brain, including the hypothalamus, the hippocampus and the spinal cord, and has many different physiological actions, depending upon its site of production. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. As such, it may help some individuals against addiction. Blocking dynorphin may help alleviate depression.
- ADCA antibody
- Beta neoendorphin dynorphin antibody
- Dyn A17 antibody
IHC image of ab40647 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40647, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab40647 has not yet been referenced specifically in any publications.