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Would like to use one of these dynorphin A antibodies in formalin-fixed, free-floating tissue sections, but these antibodies recommend an antigen retrieval step. Is there a protocol that you recommend?
Asked on Feb 22 2012
Thank you for your call yesterday and for your question about these antibodies. I apologize for the delay getting back to you.
We have a general IHC protocol for FFPE sections that includes a section on antigen retrieval-
However, these methods are optimized for slide-mounted tissue, so I have found some other resources that would be more useful for free floation sections-
I've also attached a couple references for free floating antigen retrieval. You should be able to perform this step in a 24-well plate so it will be compatible with your current staining protocol.
I hope this information will be useful, but if you have any further questions or need anything else, please let me know and I'll be happy to help you further.
Answered on Feb 22 2012