Anti-DYRK1A antibody (ab156818)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-DYRK1A antibody
See all DYRK1A primary antibodies -
DescriptionGoat polyclonal to DYRK1A
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Host speciesGoat
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Specificityab156818 is expected to recognize all reported isoforms (NP_001387.2; NP_569120.1; NP_567824.1; NP_569122.1).
ab156818 is not expected to cross-react with DYRK1B. -
Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig
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Immunogen
Synthetic peptide corresponding to Human DYRK1A aa 35-47 (N terminal) (Cysteine residue).
Sequence:PHSHQYSDRRQPN
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Positive control
- K562 cell line lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab156818 was purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab156818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 86 kDa). |
Target
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FunctionMay play a role in a signaling pathway regulating nuclear functions of cell proliferation. Phosphorylates serine, threonine and tyrosine residues in its sequence and in exogenous substrates.
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Tissue specificityUbiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney.
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Involvement in diseaseDefects in DYRK1A are the cause of mental retardation autosomal dominant type 7 (MRD7) [MIM:614104]. A disease characterized by primary microcephaly, severe mental retardation without speech, anxious autistic behavior, and dysmorphic features, including bitemporal narrowing, deep-set eyes, large simple ears, and a pointed nasal tip. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period.
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Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
Contains 1 protein kinase domain. -
Developmental stageExpressed in the developing central nervous system. Overexpressed 1.5-fold in fetal Down syndrome brain.
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DomainThe polyhistidine repeats act as targeting signals to nuclear speckles (PubMed:19266028).
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Post-translational
modificationsAutophosphorylated on tyrosine residues. -
Cellular localizationNucleus speckle.
- Information by UniProt
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Database links
- Entrez Gene: 1859 Human
- Entrez Gene: 13548 Mouse
- Entrez Gene: 25255 Rat
- Omim: 600855 Human
- SwissProt: Q13627 Human
- SwissProt: Q61214 Mouse
- SwissProt: Q63470 Rat
- Unigene: 368240 Human
see all -
Alternative names
- Dual specificity tyrosine phosphorylation regulated kinase antibody
- Dual specificity tyrosine (Y) phosphorylation regulated kinase 1A antibody
- Dual specificity tyrosine phosphorylation regulated kinase 1 antibody
see all
Images
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Western blot - Anti-DYRK1A antibody (ab156818)Anti-DYRK1A antibody (ab156818) at 0.5 µg/ml + K562 cell line lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 86 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
This product has been referenced in:
- Wang P et al. Dual-specificity tyrosine-phosphorylation regulated kinase 1A Gene Transcription is regulated by Myocyte Enhancer Factor 2D. Sci Rep 7:7240 (2017). Read more (PubMed: 28775333) »
