Anti-DYRK1A antibody (ab156818)
Key features and details
- Goat polyclonal to DYRK1A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-DYRK1A antibody
See all DYRK1A primary antibodies -
Description
Goat polyclonal to DYRK1A -
Host species
Goat -
Specificity
ab156818 is expected to recognize all reported isoforms (NP_001387.2; NP_569120.1; NP_567824.1; NP_569122.1).
ab156818 is not expected to cross-react with DYRK1B. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide corresponding to Human DYRK1A aa 35-47 (N terminal) (Cysteine residue).
Sequence:PHSHQYSDRRQPN
-
Positive control
- K562 cell line lysate
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab156818 was purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| All applications |
Mouse
Rat
Cow
Dog
Pig
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 86 kDa).
1 hour primary incubation is recommended for this product. |
| Notes |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 86 kDa). 1 hour primary incubation is recommended for this product. |
Target
-
Function
May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Phosphorylates serine, threonine and tyrosine residues in its sequence and in exogenous substrates. -
Tissue specificity
Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney. -
Involvement in disease
Defects in DYRK1A are the cause of mental retardation autosomal dominant type 7 (MRD7) [MIM:614104]. A disease characterized by primary microcephaly, severe mental retardation without speech, anxious autistic behavior, and dysmorphic features, including bitemporal narrowing, deep-set eyes, large simple ears, and a pointed nasal tip. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
Contains 1 protein kinase domain. -
Developmental stage
Expressed in the developing central nervous system. Overexpressed 1.5-fold in fetal Down syndrome brain. -
Domain
The polyhistidine repeats act as targeting signals to nuclear speckles (PubMed:19266028). -
Post-translational
modificationsAutophosphorylated on tyrosine residues. -
Cellular localization
Nucleus speckle. - Information by UniProt
-
Database links
- Entrez Gene: 1859 Human
- Entrez Gene: 13548 Mouse
- Entrez Gene: 25255 Rat
- Omim: 600855 Human
- SwissProt: Q13627 Human
- SwissProt: Q61214 Mouse
- SwissProt: Q63470 Rat
- Unigene: 368240 Human
see all -
Alternative names
- Dual specificity tyrosine phosphorylation regulated kinase antibody
- Dual specificity tyrosine (Y) phosphorylation regulated kinase 1A antibody
- Dual specificity tyrosine phosphorylation regulated kinase 1 antibody
see all
Images
-
Western blot - Anti-DYRK1A antibody (ab156818)Anti-DYRK1A antibody (ab156818) at 0.5 µg/ml + K562 cell line lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 86 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab156818 has been referenced in 1 publication.
- Wang P et al. Dual-specificity tyrosine-phosphorylation regulated kinase 1A Gene Transcription is regulated by Myocyte Enhancer Factor 2D. Sci Rep 7:7240 (2017). PubMed: 28775333