Key features and details
- Rabbit polyclonal to DYRK1A
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-DYRK1A antibody
See all DYRK1A primary antibodies
DescriptionRabbit polyclonal to DYRK1A
Specificityab65220 detects endogenous levels of total DYRK1A protein.
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human DYRK1A aa 21-70 (internal sequence).
Database link: Q13627
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab65220 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab65220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/1000.|
|WB||1/500 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).|
FunctionMay play a role in a signaling pathway regulating nuclear functions of cell proliferation. Phosphorylates serine, threonine and tyrosine residues in its sequence and in exogenous substrates.
Tissue specificityUbiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney.
Involvement in diseaseDefects in DYRK1A are the cause of mental retardation autosomal dominant type 7 (MRD7) [MIM:614104]. A disease characterized by primary microcephaly, severe mental retardation without speech, anxious autistic behavior, and dysmorphic features, including bitemporal narrowing, deep-set eyes, large simple ears, and a pointed nasal tip. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
Contains 1 protein kinase domain.
Developmental stageExpressed in the developing central nervous system. Overexpressed 1.5-fold in fetal Down syndrome brain.
DomainThe polyhistidine repeats act as targeting signals to nuclear speckles (PubMed:19266028).
modificationsAutophosphorylated on tyrosine residues.
Cellular localizationNucleus speckle.
- Information by UniProt
- Dual specificity tyrosine phosphorylation regulated kinase antibody
- Dual specificity tyrosine (Y) phosphorylation regulated kinase 1A antibody
- Dual specificity tyrosine phosphorylation regulated kinase 1 antibody
ab65220, at a 1/500 dilution, staining DYRK1A in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
All lanes : Anti-DYRK1A antibody (ab65220) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HepG2 cell extract with immunising peptide at 10 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 86 kDa
Observed band size: 86 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab65220 has been referenced in 2 publications.
- Fujita K et al. Galectin-9 suppresses the growth of hepatocellular carcinoma via apoptosis in vitro and in vivo. Int J Oncol 46:2419-30 (2015). PubMed: 25823465
- Baek KH et al. Down's syndrome suppression of tumour growth and the role of the calcineurin inhibitor DSCR1. Nature 459:1126-30 (2009). WB ; Human . PubMed: 19458618