Overview

  • Product name

    Anti-DYRK1A antibody - C-terminal
    See all DYRK1A primary antibodies
  • Description

    Rabbit polyclonal to DYRK1A - C-terminal
  • Host species

    Rabbit
  • Specificity

    ab180910 will not cross-react with other DYRK family members.
  • Tested applications

    Suitable for: ICC, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human DYRK1A (C terminal). (17 amino acid peptide; NP_001387).
    Database link: Q13627

  • Positive control

    • HeLa cell lysate; HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 10 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 86 kDa.

Target

  • Function

    May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Phosphorylates serine, threonine and tyrosine residues in its sequence and in exogenous substrates.
  • Tissue specificity

    Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney.
  • Involvement in disease

    Defects in DYRK1A are the cause of mental retardation autosomal dominant type 7 (MRD7) [MIM:614104]. A disease characterized by primary microcephaly, severe mental retardation without speech, anxious autistic behavior, and dysmorphic features, including bitemporal narrowing, deep-set eyes, large simple ears, and a pointed nasal tip. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
    Contains 1 protein kinase domain.
  • Developmental stage

    Expressed in the developing central nervous system. Overexpressed 1.5-fold in fetal Down syndrome brain.
  • Domain

    The polyhistidine repeats act as targeting signals to nuclear speckles (PubMed:19266028).
  • Post-translational
    modifications

    Autophosphorylated on tyrosine residues.
  • Cellular localization

    Nucleus speckle.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dual specificity tyrosine phosphorylation regulated kinase antibody
    • Dual specificity tyrosine (Y) phosphorylation regulated kinase 1A antibody
    • Dual specificity tyrosine phosphorylation regulated kinase 1 antibody
    • Dual specificity tyrosine phosphorylation regulated kinase 1A antibody
    • Dual specificity tyrosine-phosphorylation-regulated kinase 1A antibody
    • Dual specificity YAK 1 related kinase antibody
    • Dual specificity YAK1 related kinase antibody
    • Dual specificity YAK1-related kinase antibody
    • DYR1A_HUMAN antibody
    • DYRK 1 antibody
    • DYRK 1A antibody
    • DYRK antibody
    • DYRK1 antibody
    • Dyrk1a antibody
    • DYRKA antibody
    • hMNB antibody
    • HP 86 antibody
    • HP86 antibody
    • Minibrain (Drosophila) homolog antibody
    • Minibrain homolog antibody
    • Minibrain, Drosophila, homolog of antibody
    • MNB antibody
    • MNB protein kinase antibody
    • Mnb protein kinase homolog hp86 antibody
    • MNB protein kinase, Serine/threonine-specific antibody
    • MNB/DYRK protein kinase antibody
    • MNBH antibody
    • MRD7 antibody
    • OTTHUMP00000109090 antibody
    • OTTHUMP00000109091 antibody
    • OTTHUMP00000109094 antibody
    • OTTHUMP00000174799 antibody
    • Protein kinase minibrain homolog antibody
    • Serine/threonine kinase MNB antibody
    see all

Images

  • Western blot analysis of DYRK1A expression in K562 cell lysate with ab180910 at (A) 1 and (B) 2µg/ml.
  • Western Blot of HeLa cell lysate labeling DYRK1A with Anti-DYRK1A antibody - C-terminal (ab180910) at (1) 1 and (2) 2µg/ml.
  • All lanes : Anti-DYRK1A antibody - C-terminal (ab180910) at 1 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : Daudi cell lysate
    Lane 3 : EL4 cell lysate

    Predicted band size: 86 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?



    15ug protein per lane

    Blocking buffer: 5% non-fat dry milk in TBST

  • Immunocytochemical analysis of HeLa cells labeling DYRK1A with ab180910 at 10 µg/ml.

  • Lane 1 : Anti-DYRK1A antibody - C-terminal (ab180910) at 1 µg/ml
    Lane 2 : Anti-DYRK1A antibody - C-terminal (ab180910) at 2 µg/ml

    All lanes : HeLa cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 86 kDa

References

ab180910 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (iPSC-derived astrocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
iPSC-derived astrocytes
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C

Dr. Kate Hawkins

Verified customer

Submitted Dec 11 2018

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