Overview

  • Product name

    Anti-Dyrk1B/MIRK antibody [EPR5617]
    See all Dyrk1B/MIRK primary antibodies
  • Description

    Rabbit monoclonal [EPR5617] to Dyrk1B/MIRK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Dyrk1B/MIRK. The exact sequence is proprietary.

  • Positive control

    • HT 1080, Neuro 2a, SW480 and HeLa whole cell lysate (ab150035); Human brain tissue.
  • General notes

     This product was previously labelled as Dyrk1B

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5617
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Dual-specificity kinase which possesses both serine/ threonine and tyrosine kinase activities. Enhances the transcriptional activity of TCF1/HNF1A and FOXO1. Inhibits epithelial cell migration. Mediates colon carcinoma cell survival in mitogen-poor environments.
    • Tissue specificity

      Highest expression in skeletal muscle, testis, heart and brain with little expression in colon or lung. Expressed in a variety of tumor cell lines.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated on tyrosine residues. Phosphorylated by MAP kinase. Tyrosine phosphorylation may be required for dimerization.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • AOMS3 antibody
      • Dual specificity tyrosine (Y) phosphorylation regulated kinase 1B antibody
      • Dual specificity tyrosine phosphorylation regulated kinase 1B antibody
      • Dual specificity tyrosine-phosphorylation-regulated kinase 1B antibody
      • DYR 1B antibody
      • DYR1A antibody
      • DYR1B antibody
      • DYR1B_HUMAN antibody
      • DYRK 1B antibody
      • Dyrk1b antibody
      • Minibrain related kinase antibody
      • Minibrain-related kinase antibody
      • MIRK antibody
      • Mirk protein kinase antibody
      see all

    Images

    • All lanes : Anti-Dyrk1B/MIRK antibody [EPR5617] (ab124960) at 1/1000 dilution

      Lane 1 : HT 1080 cell lysates
      Lane 2 : Neuro 2a cell lysates
      Lane 3 : SW480 cell lysates
      Lane 4 : HeLa cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 69 kDa

    • ab124960, at 1/50 dilution staining Dyrk1B/MIRK in paraffin-embedded Human brain tissue, by Immunohistochemistry.

    References

    ab124960 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab124960.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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