Product nameAnti-Dyrk1B/MIRK antibody [EPR5617]
See all Dyrk1B/MIRK primary antibodies
DescriptionRabbit monoclonal [EPR5617] to Dyrk1B/MIRK
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Dyrk1B/MIRK. The exact sequence is proprietary.
- HT 1080, Neuro 2a, SW480 and HeLa whole cell lysate (ab150035); Human brain tissue.
This product was previously labelled as Dyrk1B
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)|
|ICC||1/100 - 1/250.|
FunctionDual-specificity kinase which possesses both serine/ threonine and tyrosine kinase activities. Enhances the transcriptional activity of TCF1/HNF1A and FOXO1. Inhibits epithelial cell migration. Mediates colon carcinoma cell survival in mitogen-poor environments.
Tissue specificityHighest expression in skeletal muscle, testis, heart and brain with little expression in colon or lung. Expressed in a variety of tumor cell lines.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
Contains 1 protein kinase domain.
modificationsAutophosphorylated on tyrosine residues. Phosphorylated by MAP kinase. Tyrosine phosphorylation may be required for dimerization.
- Information by UniProt
- AOMS3 antibody
- Dual specificity tyrosine (Y) phosphorylation regulated kinase 1B antibody
- Dual specificity tyrosine phosphorylation regulated kinase 1B antibody
All lanes : Anti-Dyrk1B/MIRK antibody [EPR5617] (ab124960) at 1/1000 dilution
Lane 1 : HT 1080 cell lysates
Lane 2 : Neuro 2a cell lysates
Lane 3 : SW480 cell lysates
Lane 4 : HeLa cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 69 kDa
ab124960, at 1/50 dilution staining Dyrk1B/MIRK in paraffin-embedded Human brain tissue, by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab124960 has not yet been referenced specifically in any publications.