Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab2248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Can be blocked with Human Dysadherin peptide (ab22894).|
FunctionInvolved in down-regulation of E-cadherin which results in reduced cell adhesion. Promotes metastasis.
Sequence similaritiesBelongs to the FXYD family.
- Information by UniProt
- Dysad antibody
- Dysadherin antibody
- FXYD 5 antibody
ab2248 staining (0.5
µg/ml) of Jurkat lysate (RIPA buffer, 30 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab2248 (0.5 µg/ml) staining Human Spleen lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
This product has been referenced in:
- Arystarkhova E et al. Multiplicity of expression of FXYD proteins in mammalian cells: dynamic exchange of phospholemman and gamma-subunit in response to stress. Am J Physiol Cell Physiol 292:C1179-91 (2007). WB ; Human . Read more (PubMed: 17050615) »