Overview

  • Product name

    Anti-Dystonin/BPA antibody
    See all Dystonin/BPA primary antibodies
  • Description

    Rabbit polyclonal to Dystonin/BPA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Dystonin/BPA aa 1-195.
    Database link: Q03001

  • Positive control

    • ICC/IF: HeLa cells. WB: Recombinant human Dystonin/BPA protein.
  • General notes

     This product was previously labelled as Dystonin

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab234644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 860 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Relevance

    Cytoskeletal linker protein. Acts as an integrator of intermediate filaments, actin and microtubule cytoskeleton networks. Required for anchoring either intermediate filaments to the actin cytoskeleton in neural and muscle cells or keratin-containing intermediate filaments to hemidesmosomes in epithelial cells. The proteins may self-aggregate to form filaments or a two-dimensional mesh. Isoform 3: plays a structural role in the assembly of hemidesmosomes of epithelial cells; anchors keratin-containing intermediate filaments to the inner plaque of hemidesmosomes. Required for the regulation of keratinocyte polarity and motility; mediates integrin ITGB4 regulation of RAC1 activity. Isoform 6: required for bundling actin filaments around the nucleus By similarity. Isoform 7: regulates the organization and stability of the microtubule network of sensory neurons to allow axonal transport.
  • Cellular localization

    Cytoplasm › cytoskeleton.
  • Database links

  • Alternative names

    • 230 kDa bullous pemphigoid antigen antibody
    • 230/240 kDa bullous pemphigoid antigen antibody
    • BP230 antibody
    • BP240 antibody
    • BPA antibody
    • BPAG1 antibody
    • Bullous pemphigoid antigen 1 antibody
    • Bullous pemphigoid antigen antibody
    • DMH antibody
    • DST antibody
    • DT antibody
    • Dystonia musculorum protein antibody
    • Hemidesmosomal plaque protein antibody
    • KIAA0728 antibody
    see all

Images

  • HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Dystonin/BPA (green) using ab234644 at 1/100 dilution in ICC/IF, followed by Alexa-Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

  • All lanes : Anti-Dystonin/BPA antibody (ab234644) at 1/500 dilution

    Lane 1 : Recombinant human Dystonin/BPA protein at 0.1 µg
    Lane 2 : Recombinant human Dystonin/BPA protein at 0.05 µg
    Lane 3 : Recombinant human Dystonin/BPA protein at 0.025 µg
    Lane 4 : Recombinant human Dystonin/BPA protein at 0.0125 µg

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 860 kDa

References

ab234644 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab234644.
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