Key features and details
- Rabbit polyclonal to Dystonin/BPA
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Dystonin/BPA antibody
See all Dystonin/BPA primary antibodies
DescriptionRabbit polyclonal to Dystonin/BPA
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Dystonin/BPA aa 1-195.
Database link: Q03001
- ICC/IF: HeLa cells. WB: Recombinant human Dystonin/BPA protein.
This product was previously labelled as Dystonin.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab234644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 860 kDa.|
|ICC/IF||1/50 - 1/200.|
RelevanceCytoskeletal linker protein. Acts as an integrator of intermediate filaments, actin and microtubule cytoskeleton networks. Required for anchoring either intermediate filaments to the actin cytoskeleton in neural and muscle cells or keratin-containing intermediate filaments to hemidesmosomes in epithelial cells. The proteins may self-aggregate to form filaments or a two-dimensional mesh. Isoform 3: plays a structural role in the assembly of hemidesmosomes of epithelial cells; anchors keratin-containing intermediate filaments to the inner plaque of hemidesmosomes. Required for the regulation of keratinocyte polarity and motility; mediates integrin ITGB4 regulation of RAC1 activity. Isoform 6: required for bundling actin filaments around the nucleus By similarity. Isoform 7: regulates the organization and stability of the microtubule network of sensory neurons to allow axonal transport.
Cellular localizationCytoplasm › cytoskeleton.
- 230 kDa bullous pemphigoid antigen antibody
- 230/240 kDa bullous pemphigoid antigen antibody
- BP230 antibody
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Dystonin/BPA (green) using ab234644 at 1/100 dilution in ICC/IF, followed by Alexa-Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
All lanes : Anti-Dystonin/BPA antibody (ab234644) at 1/500 dilution
Lane 1 : Recombinant human Dystonin/BPA protein at 0.1 µg
Lane 2 : Recombinant human Dystonin/BPA protein at 0.05 µg
Lane 3 : Recombinant human Dystonin/BPA protein at 0.025 µg
Lane 4 : Recombinant human Dystonin/BPA protein at 0.0125 µg
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 860 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234644 has not yet been referenced specifically in any publications.