Key features and details
- Rabbit polyclonal to dystrophia myotonica containing WD repeat motif
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-dystrophia myotonica containing WD repeat motif antibody
See all dystrophia myotonica containing WD repeat motif primary antibodies
DescriptionRabbit polyclonal to dystrophia myotonica containing WD repeat motif
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide corresponding to a region between residues 609 and 659 of human dystrophia myotonica containing WD repeat motif (NP_004934.1).
- HeLa and 293T whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab85932 was affinity purified using an epitope specific to dystrophia myotonica containing WD repeat motif immobilized on solid support.
Our Abpromise guarantee covers the use of ab85932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 69 kDa.|
FunctionCould have a regulatory function in meiosis.
Tissue specificityStrongest expression in brain, liver, and testis. Also expressed in kidney and spleen.
Sequence similaritiesContains 5 WD repeats.
- Information by UniProt
- D19S593E antibody
- DM 9 antibody
- DM9 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling dystrophia myotonica containing WD repeat motif with ab85932 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-dystrophia myotonica containing WD repeat motif antibody (ab85932) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 69 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
ab85932 has not yet been referenced specifically in any publications.