RecombinantRabMAb

Recombinant Anti-Dystrophin antibody [EPR21189] (ab218198)

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Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)
  • Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)
  • Anti-Dystrophin antibody [EPR21189] (ab218198)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21189] to Dystrophin
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Dystrophin antibody [EPR21189]
    See all Dystrophin primary antibodies

  • Description

    Rabbit monoclonal [EPR21189] to Dystrophin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Mouse Dystrophin aa 3650 to the C-terminus. The exact sequence is proprietary.
    Database link: P11531

  • Positive control

    • IHC-P: Mouse cardiac muscle and skeletal muscle tissue; rat colon tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab218198 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr 1/50.

Positive membrane staining of cardiomyocytes on mouse and Rat heart tissue section (PMID: 26430202).

Target

  • Function

    Anchors the extracellular matrix to the cytoskeleton via F-actin. Ligand for dystroglycan. Component of the dystrophin-associated glycoprotein complex which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems and has a structural function in stabilizing the sarcolemma. Also implicated in signaling events and synaptic transmission.

  • Tissue specificity

    Expressed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma. Expressed in brain, muscle, kidney, lung and testis. Isoform 5 is expressed in heart, brain, liver, testis and hepatoma cells. Most tissues contain transcripts of multiple isoforms, however only isoform 5 is detected in heart and liver.

  • Involvement in disease

    Defects in DMD are the cause of Duchenne muscular dystrophy (DMD) [MIM:310200]. DMD is the most common form of muscular dystrophy; a sex-linked recessive disorder. It typically presents in boys aged 3 to 7 year as proximal muscle weakness causing waddling gait, toe-walking, lordosis, frequent falls, and difficulty in standing up and climbing up stairs. The pelvic girdle is affected first, then the shoulder girdle. Progression is steady and most patients are confined to a wheelchair by age of 10 or 12. Flexion contractures and scoliosis ultimately occur. About 50% of patients have a lower IQ than their genetic expectations would suggest. There is no treatment.
    Defects in DMD are the cause of Becker muscular dystrophy (BMD) [MIM:300376]. BMD resembles DMD in hereditary and clinical features but is later in onset and more benign.
    Defects in DMD are a cause of cardiomyopathy dilated X-linked type 3B (CMD3B) [MIM:302045]; also known as X-linked dilated cardiomyopathy (XLCM). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

  • Sequence similarities

    Contains 2 CH (calponin-homology) domains.
    Contains 22 spectrin repeats.
    Contains 1 WW domain.
    Contains 1 ZZ-type zinc finger.

  • Cellular localization

    Cell membrane > sarcolemma. Cytoplasm > cytoskeleton.
  • Information by UniProt

  • Database links

    • Alternative names

      • BMD antibody
      • CMD3B antibody
      • DMD antibody
      • DMD_HUMAN antibody
      • Duchenne muscular dystrophy protein antibody
      • Dystrophin antibody
      • Muscular dystrophy Duchenne and Becker types antibody
      see all

    Images

    • Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)
      Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)

      Immunohistochemical (Frozen sections) analysis of rat heart tissue useing ab218198 (1/50 dilution) to stain Dystrophin. ab150077 AlexaFluor®488 Goat anti-Rabbit (1/500) secondary used. DAPI used as a nuclear counter stain. Heat-mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0). Fixative 4% PFA, Permeabilisation with 0.2% Triton X-100.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)

      Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Dystrophin with ab218198 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in smooth muscle of rat colon (PMID: 18806224) is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)
      Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)

      Immunohistochemical (Frozen sections) analysis of mouse heart tissue useing ab218198 (1/50 dilution) to stain Dystrophin. ab150077 AlexaFluor®488 Goat anti-Rabbit (1/500) secondary used. DAPI used as a nuclear counter stain. Heat-mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0). Fixative 4% PFA, Permeabilisation with 0.2% Triton X-100.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)

      Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Dystrophin with ab218198 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse skeletal muscle (PMID: 24793134) is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)

      Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Dystrophin with ab218198 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse cardiac muscle (PMID: 24793134) is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

       

    • Anti-Dystrophin antibody [EPR21189] (ab218198)
      Anti-Dystrophin antibody [EPR21189] (ab218198)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab218198? Please let us know so that we can cite the reference in this datasheet.

    ab218198 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Dystrophin antibody [EPR21189]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Myocardium)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Myocardium
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Mar 03 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Dystrophin antibody [EPR21189]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Myocardium)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Myocardium
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Mar 03 2020

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