Recombinant Anti-Dystrophin antibody [EPR21189] (ab218198)
![Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)](https://www.abcam.com/ps/products/218/ab218198/Images/ab218198-298596-anti-dystrophin-antibody-epr21189-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21189] to Dystrophin
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-Dystrophin antibody [EPR21189]
See all Dystrophin primary antibodies -
Description
Rabbit monoclonal [EPR21189] to Dystrophin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse cardiac muscle and skeletal muscle tissue; rat colon tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21189 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218198 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (2) |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| IHC-Fr | (1) |
1/50.
Positive membrane staining of cardiomyocytes on mouse and Rat heart tissue section (PMID: 26430202). |
| Notes |
|---|
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IHC-Fr
1/50. Positive membrane staining of cardiomyocytes on mouse and Rat heart tissue section (PMID: 26430202). |
Target
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Function
Anchors the extracellular matrix to the cytoskeleton via F-actin. Ligand for dystroglycan. Component of the dystrophin-associated glycoprotein complex which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems and has a structural function in stabilizing the sarcolemma. Also implicated in signaling events and synaptic transmission. -
Tissue specificity
Expressed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma. Expressed in brain, muscle, kidney, lung and testis. Isoform 5 is expressed in heart, brain, liver, testis and hepatoma cells. Most tissues contain transcripts of multiple isoforms, however only isoform 5 is detected in heart and liver. -
Involvement in disease
Defects in DMD are the cause of Duchenne muscular dystrophy (DMD) [MIM:310200]. DMD is the most common form of muscular dystrophy; a sex-linked recessive disorder. It typically presents in boys aged 3 to 7 year as proximal muscle weakness causing waddling gait, toe-walking, lordosis, frequent falls, and difficulty in standing up and climbing up stairs. The pelvic girdle is affected first, then the shoulder girdle. Progression is steady and most patients are confined to a wheelchair by age of 10 or 12. Flexion contractures and scoliosis ultimately occur. About 50% of patients have a lower IQ than their genetic expectations would suggest. There is no treatment.
Defects in DMD are the cause of Becker muscular dystrophy (BMD) [MIM:300376]. BMD resembles DMD in hereditary and clinical features but is later in onset and more benign.
Defects in DMD are a cause of cardiomyopathy dilated X-linked type 3B (CMD3B) [MIM:302045]; also known as X-linked dilated cardiomyopathy (XLCM). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similarities
Contains 2 CH (calponin-homology) domains.
Contains 22 spectrin repeats.
Contains 1 WW domain.
Contains 1 ZZ-type zinc finger. -
Cellular localization
Cell membrane > sarcolemma. Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 13405 Mouse
- Entrez Gene: 24907 Rat
- SwissProt: P11531 Mouse
- SwissProt: P11530 Rat
- Unigene: 275608 Mouse
- Unigene: 416750 Mouse
- Unigene: 10307 Rat
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Alternative names
- BMD antibody
- CMD3B antibody
- DMD antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)
Immunohistochemical (Frozen sections) analysis of rat heart tissue useing ab218198 (1/50 dilution) to stain Dystrophin. ab150077 AlexaFluor®488 Goat anti-Rabbit (1/500) secondary used. DAPI used as a nuclear counter stain. Heat-mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0). Fixative 4% PFA, Permeabilisation with 0.2% Triton X-100.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)](https://www.abcam.com/ps/products/218/ab218198/Images/ab218198-297872-anti-dystrophin-antibody-epr21189-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Dystrophin with ab218198 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in smooth muscle of rat colon (PMID: 18806224) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)](https://www.abcam.com/ps/products/218/ab218198/Images/ab218198-298595-anti-dystrophin-antibody-epr21189-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)Immunohistochemical (Frozen sections) analysis of mouse heart tissue useing ab218198 (1/50 dilution) to stain Dystrophin. ab150077 AlexaFluor®488 Goat anti-Rabbit (1/500) secondary used. DAPI used as a nuclear counter stain. Heat-mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0). Fixative 4% PFA, Permeabilisation with 0.2% Triton X-100.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)](https://www.abcam.com/ps/products/218/ab218198/Images/ab218198-297871-anti-dystrophin-antibody-epr21189-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Dystrophin with ab218198 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse skeletal muscle (PMID: 24793134) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)](https://www.abcam.com/ps/products/218/ab218198/Images/ab218198-297870-anti-dystrophin-antibody-epr21189-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR21189] (ab218198)Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Dystrophin with ab218198 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse cardiac muscle (PMID: 24793134) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Anti-Dystrophin antibody [EPR21189] (ab218198)](https://www.abcam.com/ps/products/218/ab218198/Images/ab218198-7-benefits-of-recombinant-antibodies.png)
Anti-Dystrophin antibody [EPR21189] (ab218198)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab218198 has been referenced in 1 publication.
- Meyers TA et al. DMD carrier model with mosaic dystrophin expression in the heart reveals complex vulnerability to myocardial injury. Hum Mol Genet 29:944-954 (2020). PubMed: 31976522
