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I'm interested in the ab7164 mandra-1 dystrophin antibody. However, I've one question, this is written in the datasheet 'The epitope recognized by the antibody is sensitive to formalin fixation and paraffin embedding'. I want to use this antibody on fixed cells and wonder if pfa fixation is suitable in this case. If not, what do you recommend me?
Asked on Feb 01 2006
Thank you for your enquiry. The antibody will work in frozen sections and I would recommend the following references as sources of staining protocols as they have tested the antibody in IHC: Ellis JM et al. Specificity of dystrophin analysis improved with monoclonal antibodies. Lancet 336:881-2 (1990). PubMed: 1699095. Nguyen TM et al. Monoclonal antibodies for dystrophin analysis. Epitope mapping and improved binding to SDS-treated muscle sections. Biochem J 288 (Pt 2):663-8 (1992). PubMed: 1281410 Gussoni E et al. Normal dystrophin transcripts detected in Duchenne muscular dystrophy patients after myoblast transplantation. Nature 356:435-8 (1992). PubMed: 1557125 I hope these references will help, please let me know if you need further information,
Answered on Feb 02 2006