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Thank you for your very efficient dealingwith my concern regarding the nNOS antibody (see below) for frozen tissue sections. We did get in exchange the otherantibody I had referred to, and it is working both for frozen sections and western blots. The MHC II antibody for western blots, that wepurchased around the same time, also worked very well. So, we will continue orderingthese antibodies.
However,we have a serious problem now with the dystrophin antibody MANDRA1, ab7164 which you advertise for western blots.In our hands, itdid not work for about 80 ug protein from myofibers in wild type mouse skeletal muscle homogenates, where MHC II is working very fine, even with 0.5 ug protein.
I would like touse what we paid for the MANDRA1 (ab7164) as a credit for testing an alternative antibody. I have identified ab15277 (rabbit polyclonal) as of potential interest, even if no western blot is shown (although the recommended dilution is 1/100), because it seems to be widely used. The alternative seems to be ab3149 (mouse monoclonal). It does show a nice band, but it does not tell whether it isfrom a skeletal muscle extract, what amount of protein and what antibody dilution.
Can you please e-mail me to advise on any of these or alternative antibodies for mouse dystrophin in western blot of skeletal muscle homogenates. I would like to talk to you on the phone.
Asked on Jan 18 2012
Thank you for your email. It was nice to speak to you over the phone.
I am sorry to hear that you are encountering problems with ab7164.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1017104 with ab3149.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.
Answered on Jan 18 2012