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I get staining in my cells when treated with the primary antibody but no secondary antibody.
Asked on May 22 2012
Thank you for your phone call. As we discussed, this antibody is not conjugated so it should not produce any signal in the absence of a secondary antibody. It is likely that the signal you are observing is autofluorescence. The attached reference details several common types of autofluorescence and tips for avoiding them. I hope this helps, please let me know if you have any additional questions.
Answered on May 22 2012