Western blot abreview for Anti-Dystrophin antibody [MANDRA1]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse myotubes)
Loading amount
30 µg
Specification
mouse myotubes
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/300
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA

Secondary antibody

Dilution
1/8000

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 427 kDa

Additional data

Additional Notes
line 1 - whole lysate from mouse myotubes

Nice clear band on expected size. No additional unspecific bands. Unfortunately this antibody didn't detect Dp71 in my samples, but it was not very important for me. Overall very good.

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Submitted Jul 21 2011

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