Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse myotubes)
Loading amount
30 µg
Specification
mouse myotubes
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/300
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Secondary antibody
Name
Dilution
1/8000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 427 kDa
Additional data
Additional Notes
line 1 - whole lysate from mouse myotubes
Nice clear band on expected size. No additional unspecific bands. Unfortunately this antibody didn't detect Dp71 in my samples, but it was not very important for me. Overall very good.
Nice clear band on expected size. No additional unspecific bands. Unfortunately this antibody didn't detect Dp71 in my samples, but it was not very important for me. Overall very good.
Abcam user community
Verified customer
Submitted Jul 21 2011