Key features and details
- Rat monoclonal [DECMA-1] to E Cadherin - Intercellular Junction Marker
- Suitable for: ICC/IF, Flow Cyt, WB, IHC-Fr
- Reacts with: Mouse, Dog
- Isotype: IgG1
Product nameAnti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker
See all E Cadherin primary antibodies
DescriptionRat monoclonal [DECMA-1] to E Cadherin - Intercellular Junction Marker
Some customers have used this antibody successfully in human; however, our latest tests were unsuccessful and therefore we can no longer guarantee the reactivity of this species.
Tested applicationsSuitable for: ICC/IF, Flow Cyt, WB, IHC-Frmore details
Species reactivityReacts with: Mouse, Dog
Predicted to work with: Cow
corresponding to E Cadherin.
- ICC/IF: M158 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab11512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 - 10 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 20521328
ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||Use at an assay dependent concentration. PubMed: 19586906|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionCadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
Tissue specificityNon-neural epithelial tissues.
Involvement in diseaseDefects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
Defects in CDH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
Defects in CDH1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
Sequence similaritiesContains 5 cadherin domains.
modificationsDuring apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
Cellular localizationCell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
- Information by UniProt
- Arc 1 antibody
- CADH1_HUMAN antibody
- Cadherin 1 antibody
ab11512 staining E Cadherin in Mouse embryonic (E14.5) lung tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% serum for 3 hours at 4°C. Samples were incubated with primary antibody (1/500 in blocking buffer) for 14 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rat IgG polyclonal (1/200) was used as the secondary antibody.
ab11512 stained in M158 cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab11512 at 10 µg/ml overnight at +4°C. The secondary antibody was ab150165 (colored green) used at 1 µg/ml for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.
Sample: Murine derived breast cancer whole cell lysate, 30 µg.
ab11512 used at a 1/5000 dilution for 16 hours at 4°C.
Goat polyclonal IRDye 800CW used as the secondary antibody at a 1/10,000 dilution.
ab11512 E Cadherin staining canine kidney (MDCK) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 37°C. Samples were incubated with primary antibody, 1/100, in blocking buffer for 1 hour at 37°C. An undiluted Cy3®-conjugated Donkey polyclonal to rat IgG was used as secondary antibody.
ab11512 at 1/500 staining dog kidney cells by ICC/IF. The cells were methanol fixed and blocked with BSA before incubation with the antibody for 18 hours at 4°C. An Alexa Fluor ® 555 conjugated goat anti-rat IgG was used as the secondary.
ab11512 has been referenced in 89 publications.
- Chow T et al. Recapitulating kidney development in vitro by priming and differentiating mouse embryonic stem cells in monolayers. NPJ Regen Med 5:7 (2020). PubMed: 32351711
- Leng S et al. ß-Catenin and FGFR2 regulate postnatal rosette-based adrenocortical morphogenesis. Nat Commun 11:1680 (2020). PubMed: 32245949
- Li Q et al. Donkey milk inhibits triple-negative breast tumor progression and is associated with increased cleaved-caspase-3 expression. Food Funct 11:3053-3065 (2020). PubMed: 32191229
- Thompson EA et al. Interstitial Migration of CD8aß T Cells in the Small Intestine Is Dynamic and Is Dictated by Environmental Cues. Cell Rep 26:2859-2867.e4 (2019). PubMed: 30865878
- Defourny J et al. EphA4-ADAM10 Interplay Patterns the Cochlear Sensory Epithelium through Local Disruption of Adherens Junctions. iScience 11:246-257 (2019). PubMed: 30639848