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  1. Link

    e-cadherin-antibody-decma-1-intercellular-junction-marker-ab11512.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins
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Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker (ab11512)

  • Datasheet
  • SDS
Reviews (26)Q&A (9)References (122)

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Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker (ab11512)

    Key features and details

    • Rat monoclonal [DECMA-1] to E Cadherin - Intercellular Junction Marker
    • Suitable for: ICC/IF
    • Reacts with: Mouse
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker
      See all E Cadherin primary antibodies
    • Description

      Rat monoclonal [DECMA-1] to E Cadherin - Intercellular Junction Marker
    • Host species

      Rat
    • Specificity

      Some customers have used this antibody successfully in human; however, our latest tests were unsuccessful and therefore we can no longer guarantee the reactivity of this species.

    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Mouse
      Predicted to work with: Cow, Dog
    • Immunogen

      Full length protein. This information is proprietary to Abcam and/or its suppliers.

    • Positive control

      • ICC/IF: M158 cells.
    • General notes

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 6.97% L-Arginine
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      DECMA-1
    • Isotype

      IgG1
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cell Adhesion
      • Cadherins
      • Signal Transduction
      • Signaling Pathway
      • Calcium Signaling
      • Calcium Binding Proteins
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Cell adhesion
      • Cadherins
      • Developmental Biology
      • Organogenesis
      • Excretory system development
      • Kidney development
      • Neuroscience
      • Development

    Associated products

    • Alternative Versions

      • Anti-E Cadherin antibody [4A2] (ab231303)
      • Anti-E Cadherin antibody [DECMA-1] - BSA and Azide free (ab264106)
    • Compatible Secondaries

      • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
      • Goat Anti-Rat IgG H&L (HRP) (ab205720)
    • Isotype control

      • Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)
    • Related Products

      • PP2, Src and RIP2 kinase inhibitor (ab120308)
      • PP3, Negative control for PP2 (ab120617)
      • Src Kinase Inhibitor I (SKI-1) (ab120839)
      • PP 1, Src family tyrosine kinase inhibitor (ab120859)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab11512 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF (11)
    Use a concentration of 5 µg/ml.
    Notes
    ICC/IF
    Use a concentration of 5 µg/ml.

    Target

    • Function

      Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
      E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
    • Tissue specificity

      Non-neural epithelial tissues.
    • Involvement in disease

      Defects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
      Defects in CDH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
      Defects in CDH1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    • Sequence similarities

      Contains 5 cadherin domains.
    • Post-translational
      modifications

      During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
    • Cellular localization

      Cell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
    • Target information above from: UniProt accession P12830 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 282637 Cow
      • Entrez Gene: 12550 Mouse
      • SwissProt: Q6R8F2 Cow
      • SwissProt: P09803 Mouse
      • Unigene: 35605 Mouse
      • Alternative names

        • Arc 1 antibody
        • CADH1_HUMAN antibody
        • Cadherin 1 antibody
        • cadherin 1 type 1 E-cadherin antibody
        • Cadherin-1 antibody
        • Cadherin1 antibody
        • CAM 120/80 antibody
        • CD 324 antibody
        • CD324 antibody
        • CD324 antigen antibody
        • cdh1 antibody
        • CDHE antibody
        • E-Cad/CTF3 antibody
        • E-cadherin antibody
        • ECAD antibody
        • Epithelial cadherin antibody
        • epithelial calcium dependant adhesion protein antibody
        • LCAM antibody
        • Liver cell adhesion molecule antibody
        • UVO antibody
        • Uvomorulin antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker (ab11512)
        Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker (ab11512)

        ab11512 staining E Cadherin - Intercellular Junction Marker in M158 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab11512 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150165, Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

        Also suitable in cells fixed with 100% methanol (5 min).

      Protocols

      • Whole mount IHC with ab11512

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (122)

      Publishing research using ab11512? Please let us know so that we can cite the reference in this datasheet.

      ab11512 has been referenced in 122 publications.

      • Li X  et al. Tumor-associated macrophages secret exosomal miR-155 and miR-196a-5p to promote metastasis of non-small-cell lung cancer. Transl Lung Cancer Res 10:1338-1354 (2021). PubMed: 33889514
      • Castro-Córdova P  et al. Entry of spores into intestinal epithelial cells contributes to recurrence of Clostridioides difficile infection. Nat Commun 12:1140 (2021). PubMed: 33602902
      • Xiong G  et al. Circular RNA_0074027 participates in cell proliferation, apoptosis and metastasis of colorectal cancer cells through regulation of miR-525-3p. Mol Med Rep 23:N/A (2021). PubMed: 33760126
      • Fazio M  et al. SATB2 induction of a neural crest mesenchyme-like program drives melanoma invasion and drug resistance. Elife 10:N/A (2021). PubMed: 33527896
      • Moran-Horowich A & Lemos DR Methods for the Study of Renal Fibrosis in Human Pluripotent Stem Cell-Derived Kidney Organoids. Methods Mol Biol 2299:435-445 (2021). PubMed: 34028759
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 35 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Rat Cell (Rat hepatocytes)
      Permeabilization
      Yes - 0.2% Triton X-100 in PBS
      Specification
      Rat hepatocytes
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More

      Dr. Armen Petrosyan

      Verified customer

      Submitted Apr 07 2022

      Immunocytochemistry/ Immunofluorescence abreview for Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Pig Cell (embryo)
      Permeabilization
      Yes - TritonX-100
      Specification
      embryo
      Blocking step
      BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 21 2021

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (E6.5 mouse embryo)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9 and Citric Acid Buffer pH 6
      Permeabilization
      Yes - PBT
      Specification
      E6.5 mouse embryo
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.5%
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jun 29 2021

      Immunocytochemistry/ Immunofluorescence abreview for Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (murine RM1 prostate cancer cells)
      Permeabilization
      Yes - 0.2% Triton X-100 in PBS
      Specification
      murine RM1 prostate cancer cells
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More

      Dr. Armen Petrosyan

      Verified customer

      Submitted Mar 17 2021

      Immunocytochemistry/ Immunofluorescence abreview for Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (184A1 mammary epithelial)
      Permeabilization
      Yes - PBS + 0.25% Triton-X
      Specification
      184A1 mammary epithelial
      Blocking step
      (agent) for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 19 2020

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Pig Tissue sections (Liver)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
      Permeabilization
      Yes - 0.05%Tween-20
      Specification
      Liver
      Blocking step
      Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jun 30 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rabbit Tissue sections (Liver)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6.0
      Permeabilization
      Yes - 0.05%Tween-20
      Specification
      Liver
      Blocking step
      Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jun 25 2020

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Mouse Tissue sections (Lung)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
      Permeabilization
      Yes - 0.05%Tween-20 in PBS
      Specification
      Lung
      Blocking step
      Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 26 2020

      Immunocytochemistry/ Immunofluorescence abreview for Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (lung)
      Permeabilization
      No
      Specification
      lung
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 28 2018

      Immunocytochemistry/ Immunofluorescence abreview for Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Dog Cell (MDCK)
      Permeabilization
      Yes - 0.5% Triton X-100
      Specification
      MDCK
      Blocking step
      Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
      Fixative
      10%NBF
      Read More

      MR. David Ivancic

      Verified customer

      Submitted Nov 22 2017

      1-10 of 35 Abreviews or Q&A

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