Immunohistochemistry (Frozen sections) abreview for Anti-E Cadherin antibody [HECD-1]

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Normal Skin)
Specification
Normal Skin
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C

Other product details

Dilution
1/50
Incubation time
30 minute(s) · Temperature: 21°C · Diluent: PBT (PBS + 0.5% Tween-20 + 0.5% BSA)

Secondary antibody

Name
Non-Abcam antibody was used: Polymer Detection System
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
Antibody concentration can be increased.

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Submitted Jan 11 2011

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