Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-E Cadherin antibody [HECD-1]

Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
EnVision Flex Peroxidase-blocking reagent (ready-to-use - DAKO) as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample
Cow Tissue sections (Fetal Heart)
Specification
Fetal Heart
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Diluent: PBS
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: EnVision Flex HRP (DAKO)
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
Unfortunetaly, this antibody did not work in the cow tissue.
Abcam response
This product has not been tested with cow samples so we are unsure about the cross reactivity. We will look forward to receive more data for confirming the non-reactivity with cow species.

Dr. Juliana Shimara Pires Ferrão

Verified customer

Submitted Nov 26 2014

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