Western blot abreview for Anti-E Cadherin antibody [HECD-1]

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Purified protein (N/A)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Treatment
cleavage assay
Specification
N/A
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/100

Secondary antibody

Dilution
1/3000
Name
Non-Abcam antibody was used: anti-mouse IgG IRDye 800
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye­ 800CW

Detection

Detection method
LI-COR
Exposure
2 minute(s) and 0 second(s)

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Submitted Nov 02 2017

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