Western blot abreview for Anti-E Cadherin antibody [HECD-1]

Good
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (Mice hepatocytes - plasma membrane fraction)
Gel Running Conditions
Reduced Denaturing (4-15% SDS PAGE)
Loading amount
20 µg
Specification
Mice hepatocytes - plasma membrane fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS
Dilution
1/2000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Donkey anti-Ms
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: Around 95 kDa Non-specific: 72 and 55 kDa

Additional data

Abcam response
We thank this researcher for this review of our E Cadherin antibody [HECD-1]. We have recommended against using this antibody for Mouse samples because of the background bands this researcher has observed. Nevertheless, we appreciate the demonstration of positive signal in these samples.

Dr. Armen Petrosyan

Verified customer

Submitted Apr 25 2018

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