Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-E Cadherin antibody [HECD-1]

Poor
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (mammary gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate ph 6,0
Permeabilization
No
Specification
mammary gland
Blocking step
Background Block (CELL MARQUE) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA 1% PBS buffer
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: LSAB2 System-HRP DAKO
Host species: Goat
Clonality: Polyclonal
Conjugation: Streptavidin

Additional data

Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this species. We encourage all our customers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.

Prof. ALESSANDRA MEDEIROS

Verified customer

Submitted Oct 15 2018

Sign up