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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins
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Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)

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  • SDS
Reviews (42)Q&A (22)References (458)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
  • Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
  • Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
  • Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
  • Flow Cytometry - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
  • Flow Cytometry - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)

Key features and details

  • Mouse monoclonal [HECD-1] to E Cadherin - Intercellular Junction Marker
  • Suitable for: IHC-P, Flow Cyt, WB
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker
    See all E Cadherin primary antibodies
  • Description

    Mouse monoclonal [HECD-1] to E Cadherin - Intercellular Junction Marker
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Breast tumor cell line MCF-7

  • Positive control

    • WB: MDA-MB-468 and LNCaP whole cell lysates; MCF7 cell lysate. IHC-P: Breast cancer tissue; Human stomach tissue. Flow Cyt: MCF7 and A431 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% Proprietary component
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    HECD-1
  • Myeloma

    unknown
  • Isotype

    IgG1
  • Light chain type

    unknown
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins
    • Developmental Biology
    • Organogenesis
    • Excretory system development
    • Kidney development

Associated products

  • Alternative Versions

    • Anti-E Cadherin antibody [4A2] (ab231303)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human E Cadherin protein (ab235682)
  • Related Products

    • Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker (ab11512)
    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-pan Cadherin antibody [mAbcam22744] (ab22744)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab1416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (8)
Use at an assay dependent concentration.
Flow Cyt (1)
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB (15)
1/50.
Notes
IHC-P
Use at an assay dependent concentration.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
1/50.

Target

  • Function

    Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
    E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
  • Tissue specificity

    Non-neural epithelial tissues.
  • Involvement in disease

    Defects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
    Defects in CDH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
    Defects in CDH1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
  • Sequence similarities

    Contains 5 cadherin domains.
  • Post-translational
    modifications

    During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
  • Cellular localization

    Cell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
  • Target information above from: UniProt accession P12830 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 999 Human
    • Omim: 192090 Human
    • SwissProt: P12830 Human
    • Unigene: 461086 Human
    • Alternative names

      • Arc 1 antibody
      • CADH1_HUMAN antibody
      • Cadherin 1 antibody
      • cadherin 1 type 1 E-cadherin antibody
      • Cadherin-1 antibody
      • Cadherin1 antibody
      • CAM 120/80 antibody
      • CD 324 antibody
      • CD324 antibody
      • CD324 antigen antibody
      • cdh1 antibody
      • CDHE antibody
      • E-Cad/CTF3 antibody
      • E-cadherin antibody
      • ECAD antibody
      • Epithelial cadherin antibody
      • epithelial calcium dependant adhesion protein antibody
      • LCAM antibody
      • Liver cell adhesion molecule antibody
      • UVO antibody
      • Uvomorulin antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)

      Immunohistochemical analysis of human breast cancer tissue labeling E Cadherin with ab1416.

    • Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
      Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)This image is courtesy of an Abreview submitted by Dr. Armen Petrosyan.
      Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416) at 1/50 dilution + LNCaP (human prostate cancer cell line) whole cell lyate

      See Abreview

    • Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
      Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)This image is courtesy of an Abreview submitted by Dr Alban Gaultier
      All lanes : Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416) at 1/1000 dilution

      All lanes : MDA-MB-468 cells whole cell lysate

      Lysates/proteins at 80 µg/ml per lane.

      Secondary
      All lanes : HRP-conjugated polyclonal donkey anti-mouse IgG

      Performed under reducing conditions.

      Observed band size: 110 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds

      See Abreview

    • Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
      Western blot - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
      Lanes 1-2 : Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416) at 1/50 dilution
      Lanes 3-4 : Anti-GAPDH at 1 µg/ml

      Lanes 1 & 3 : MDA-MB-231 cell lysate
      Lanes 2 & 4 : MCF-7 cell lysate


      Blocking buffer: 3% milk

    • Flow Cytometry - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
      Flow Cytometry - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)

      Overlay histogram showing MCF7 cells stained with ab1416 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1416, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    • Flow Cytometry - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
      Flow Cytometry - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)This image is ccourtesy of anonymous Abreview.

      Flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cell line labeling E Cadherin with ab1416 at 1/100 dilution (2) compared with an isotype control (1).

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)This image is a courtesy of Anonymous Abreview

      ab1416 staining E Cadherin in human stomach tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in Citrate pH 6.0 and then blocking with 5% serum for 1 hour at 23°C was performed. The primary antibody was diluted 1/100 and incubated with sample for 1 hour at 23°C. A HRP conjugated goat polyclonal to mouse IgG was used undiluted as secondary antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker (ab1416)

      Immunohistochemistry of human breast carcinoma staining E Cadherin with ab1416 at 5μg/ml

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (458)

    Publishing research using ab1416? Please let us know so that we can cite the reference in this datasheet.

    ab1416 has been referenced in 458 publications.

    • Wu Y  et al. IL-17 predicts the effect of TACE combined with apatinib in hepatocellular carcinoma. Clin Hemorheol Microcirc 77:37-47 (2021). PubMed: 32651310
    • Ding X  et al. MiR-137-3p Inhibits Colorectal Cancer Cell Migration by Regulating a KDM1A-Dependent Epithelial-Mesenchymal Transition. Dig Dis Sci N/A:N/A (2020). PubMed: 32749639
    • Li W  et al. HOXC13-AS accelerates cell proliferation and migration in oral squamous cell carcinoma via miR-378g/HOXC13 axis. Oral Oncol 111:104946 (2020). PubMed: 32763778
    • Wang J  et al. The proliferative and anti-apoptosis functions of KGF/KGFR contributes to bronchial epithelial repair in asthma. Pulm Pharmacol Ther 63:101931 (2020). PubMed: 32818548
    • He Q  et al. Long non-coding RNA UCA1 upregulates KIF20A expression to promote cell proliferation and invasion via sponging miR-204 in cervical cancer. Cell Cycle 19:2486-2495 (2020). PubMed: 32835591
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

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    1-10 of 42 Abreviews

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cow Tissue sections (Fetal Heart)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    No
    Specification
    Fetal Heart
    Blocking step
    EnVision Flex Peroxidase-blocking reagent (ready-to-use - DAKO) as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Dr. Juliana Shimara Pires Ferrão

    Verified customer

    Submitted Nov 26 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mice hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Mice hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 23 2020

    Western blot abreview for Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (liver)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (6%)
    Loading amount
    20 µg
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 06 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: PH=6 ,citrate buffer
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 18 2020

    Western blot abreview for Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human skin sample)
    Gel Running Conditions
    Reduced Denaturing (8% SDS page gel)
    Loading amount
    30 µg
    Treatment
    30Gy irradiation for 24hr
    Specification
    Human skin sample
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 18 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (mammary gland)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate ph 6,0
    Permeabilization
    No
    Specification
    mammary gland
    Blocking step
    Background Block (CELL MARQUE) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Prof. ALESSANDRA MEDEIROS

    Verified customer

    Submitted Oct 15 2018

    Western blot abreview for Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (Mice hepatocytes - plasma membrane fraction)
    Gel Running Conditions
    Reduced Denaturing (4-15% SDS PAGE)
    Loading amount
    20 µg
    Specification
    Mice hepatocytes - plasma membrane fraction
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Armen Petrosyan

    Verified customer

    Submitted Apr 25 2018

    Western blot abreview for Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Purified protein (N/A)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Treatment
    cleavage assay
    Specification
    N/A
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 02 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (LNCaP - prostate cancer cells)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    LNCaP - prostate cancer cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Armen Petrosyan

    Verified customer

    Submitted Sep 26 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Rat hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Rat hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Armen Petrosyan

    Verified customer

    Submitted Sep 26 2017

    1-10 of 42 Abreviews

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