Western blot abreview for Anti-E Cadherin antibody [HECD-1] - Intercellular Junction Marker

Western blot

Mouse Tissue lysate - other (Mice hepatocytes - plasma membrane fraction)

Reduced Denaturing (4-15% SDS PAGE)

20 µg

Mice hepatocytes - plasma membrane fraction

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS

1/2000

1/10000

Non-Abcam antibody was used: Donkey anti-Ms
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

ECL+

1 minute(s) and 0 second(s)

Specific: Around 95 kDa Non-specific: 72 and 55 kDa

We thank this researcher for this review of our E Cadherin antibody [HECD-1]. We have recommended against using this antibody for Mouse samples because of the background bands this researcher has observed. Nevertheless, we appreciate the demonstration of positive signal in these samples.