Application
Western blot
Sample
Dog Cell lysate - other (MDCK cells)
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Loading amount
10 µg
Specification
MDCK cells
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 21°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: Odyssey Blocking Buffer:TBS (1:1)
Dilution
1/250
Secondary antibody
Dilution
1/20000
Name
Non-Abcam antibody was used: donkey anti-rabbit IR680
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680RD
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680RD
Detection
Detection method
LiCor, fluorescence
Exposure
30 second(s)
Bands
Specific: ~120 kDa
Abcam user community
Verified customer
Submitted Jan 03 2019