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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins
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Anti-E Cadherin antibody [M168] - C-terminal (ab76055)

  • Datasheet
  • SDS
Reviews (17)Q&A (6)References (247)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [M168] - C-terminal (ab76055)
  • Western blot - Anti-E Cadherin antibody [M168] - C-terminal (ab76055)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [M168] - C-terminal (ab76055)
  • Immunocytochemistry - Anti-E Cadherin antibody [M168] - C-terminal (ab76055)
  • Flow Cytometry - Anti-E Cadherin antibody [M168] - C-terminal (ab76055)

Key features and details

  • Mouse monoclonal [M168] to E Cadherin - C-terminal
  • Suitable for: Flow Cyt, IHC-P, ICC, WB
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-E Cadherin antibody [M168] - C-terminal
    See all E Cadherin primary antibodies
  • Description

    Mouse monoclonal [M168] to E Cadherin - C-terminal
  • Host species

    Mouse
  • Specificity

    ab76055 does not cross react with VE Cadherin or N Cadherin.

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ICC, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Mouse E Cadherin (C terminal).

  • Positive control

    • WB: Human A431 cell lysate; IHC-P: Human colon tissue; ICC: A431 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    M168
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins
    • Developmental Biology
    • Organogenesis
    • Excretory system development
    • Kidney development
    • Signal Transduction
    • Antibodies
    • e cadherin

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human E Cadherin protein (ab235682)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P (8)
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
Use at an assay dependent concentration.
WB (4)
1/100 - 1/1000. Predicted molecular weight: 97 kDa.
Notes
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
Use at an assay dependent concentration.
WB
1/100 - 1/1000. Predicted molecular weight: 97 kDa.

Target

  • Function

    Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
    E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
  • Tissue specificity

    Non-neural epithelial tissues.
  • Involvement in disease

    Defects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
    Defects in CDH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
    Defects in CDH1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
  • Sequence similarities

    Contains 5 cadherin domains.
  • Post-translational
    modifications

    During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
  • Cellular localization

    Cell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
  • Target information above from: UniProt accession P12830 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 999 Human
    • Omim: 192090 Human
    • SwissProt: P12830 Human
    • Unigene: 461086 Human
    • Alternative names

      • Arc 1 antibody
      • CADH1_HUMAN antibody
      • Cadherin 1 antibody
      • cadherin 1 type 1 E-cadherin antibody
      • Cadherin-1 antibody
      • Cadherin1 antibody
      • CAM 120/80 antibody
      • CD 324 antibody
      • CD324 antibody
      • CD324 antigen antibody
      • cdh1 antibody
      • CDHE antibody
      • E-Cad/CTF3 antibody
      • E-cadherin antibody
      • ECAD antibody
      • Epithelial cadherin antibody
      • epithelial calcium dependant adhesion protein antibody
      • LCAM antibody
      • Liver cell adhesion molecule antibody
      • UVO antibody
      • Uvomorulin antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [M168] - C-terminal (ab76055)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [M168] - C-terminal (ab76055)Shah et al PLoS One. 2016 Jan 29;11(1):e0148018. doi: 10.1371/journal.pone.0148018. eCollection 2016. Fig 2. Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/

      Immunohistochemistry staining of E Cadherin in human MMCR (Muller’s muscle conjunctival resection) tissue.

      Xylene and rehydration with serial ethanol dilutions were used for deparaffinization. Slides were washed twice for 5 minutes in 0.25% Triton X-100 for permeabilization and blocked for 2 hours at room temperature with 2% BSA and 10% normal donkey serum in PBS. Slides were incubated overnight at 4°C with ab76055. The next day, the slides were washed twice for 5 minutes in PBS and incubated for 1–2 hours with respective secondary antibodies diluted in blocking solution (1:200–800). Vecta shield mounting medium with 4′,6-diamidino-2-phenylindole (DAPI) was placed over the slides and covered with a glass coverslip. Slides were analyzed using the Zeiss LSM 710 Confocal Microscope.

    • Western blot - Anti-E Cadherin antibody [M168] - C-terminal (ab76055)
      Western blot - Anti-E Cadherin antibody [M168] - C-terminal (ab76055)
      Lane 1 : Anti-E Cadherin antibody [M168] - C-terminal (ab76055) at 1/1000 dilution
      Lane 2 : Anti-E Cadherin antibody [M168] - C-terminal (ab76055) at 1/2000 dilution
      Lane 3 : Anti-E Cadherin antibody [M168] - C-terminal (ab76055) at 1/4000 dilution

      All lanes : A431 (Human epidermoid carcinoma cell line) denatured whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 97 kDa


      Exposure time: 2 minutes


      Blocked and probed in the presence of 5% milk, and the image was captured using chemiluminescence and film.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [M168] - C-terminal (ab76055)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [M168] - C-terminal (ab76055)

      IHC image of E Cadherin staining in human colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F.

      The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer pH 6, for 20 minutes. The section was then incubated with ab76055, 5 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunocytochemistry - Anti-E Cadherin antibody [M168] - C-terminal (ab76055)
      Immunocytochemistry - Anti-E Cadherin antibody [M168] - C-terminal (ab76055)

      ICC/IF image of ab76055 stained A431 (Human epidermoid carcinoma cell line) cells.

      The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76055, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

    • Flow Cytometry - Anti-E Cadherin antibody [M168] - C-terminal (ab76055)
      Flow Cytometry - Anti-E Cadherin antibody [M168] - C-terminal (ab76055)

      Overlay histogram showing A431 (Human epidermoid carcinoma cell line) cells stained with ab76055 (red line).

      The cells were fixed with 4% paraformaldehyde (10 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76055, 1/100 dilution) for 30 minutes at 22ºC. The secondary antibody used was a DyLight® 488 goat anti-mouse IgG (H+L) ab96879 at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions.

      Acquisition of >5,000 events was performed.

      This antibody gave a positive signal in A431 cells fixed with 80% methanol (5 minutes)/permeabilized with 0.1% PBS-Tween for 20 minutes used under the same conditions.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (247)

    Publishing research using ab76055? Please let us know so that we can cite the reference in this datasheet.

    ab76055 has been referenced in 247 publications.

    • Shen E  et al. MicroRNA-93-5p promotes epithelial-mesenchymal transition in gastric cancer by repressing tumor suppressor AHNAK expression. Cancer Cell Int 20:76 (2020). PubMed: 32190000
    • Wu X  et al. Renal protection of rhein against 5/6 nephrectomied-induced chronic kidney disease: role of SIRT3-FOXO3a signalling pathway. J Pharm Pharmacol 72:699-708 (2020). PubMed: 32196681
    • Dong Y  et al. Quercetin Inhibits the Proliferation and Metastasis of Human Non-Small Cell Lung Cancer Cell Line: The Key Role of Src-Mediated Fibroblast Growth Factor-Inducible 14 (Fn14)/ Nuclear Factor kappa B (NF-?B) pathway. Med Sci Monit 26:e920537 (2020). PubMed: 32225128
    • Wu M  et al. Blockade of the mTOR signaling pathway with rapamycin ameliorates aristolochic acid nephropathy. Exp Ther Med 19:2887-2894 (2020). PubMed: 32256773
    • Jin Z  et al. Celecoxib, a selective COX-2 inhibitor, markedly reduced the severity of tamoxifen-induced adenomyosis in a murine model. Exp Ther Med 19:3289-3299 (2020). PubMed: 32266025
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 23 Abreviews or Q&A

    Question

    What is the concentration of this antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 29 2013

    Answer

    The antibody ab76055 is provided at 0.25 mg/mL concentration.

    Read More

    Caitlin Valued Customer

    Abcam Scientific Support

    Answered on Jul 29 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-E Cadherin [M168] antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
    Sample
    Human Cell (mda mb 468 breast adenocarcinoma)
    Specification
    mda mb 468 breast adenocarcinoma
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Mr. Eli Raveh

    Verified customer

    Submitted Jan 16 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-E Cadherin antibody [M168] - C-terminal

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Catshark Tissue sections (Whole embryo section)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: OmniPrep (Zytomed)
    Permeabilization
    Yes - TritonX100 0.25% or Tween20 0.1%
    Specification
    Whole embryo section
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Rami Reshef

    Verified customer

    Submitted Mar 18 2019

    Western blot abreview for Anti-E Cadherin antibody [M168] - C-terminal

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Chicken Cell lysate - whole cell (Chicken gut crypt cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% SDS PAGE)
    Loading amount
    50 µg
    Specification
    Chicken gut crypt cells
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 15 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-E Cadherin antibody [M168]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Tammar wallaby Tissue sections (Testis- Day 1 pouch young and Day 10 PY)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris
    Permeabilization
    No
    Specification
    Testis- Day 1 pouch young and Day 10 PY
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 03 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-E Cadherin antibody [M168]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rabbit Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    Protein Block serum free from Dako as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 29 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-E Cadherin antibody [M168]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Keratinocyte (HaCaT))
    Permeabilization
    Yes - 0.2% Triton x100
    Specification
    Keratinocyte (HaCaT)
    Fixative
    Paraformaldehyde
    Read More

    Dr. Ann Wheeler

    Verified customer

    Submitted May 17 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-E Cadherin antibody [M168]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6
    Permeabilization
    No
    Specification
    Skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 10 2017

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-E Cadherin antibody [M168]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Jaculus jaculus Tissue sections (intestine)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: ProK
    Permeabilization
    Yes - 0.1% Triton
    Specification
    intestine
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Mai Tran

    Verified customer

    Submitted Oct 28 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-E Cadherin antibody [M168]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
    Permeabilization
    Yes - 0.1% Tween 20 in PBS
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 40 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Miss. Chia-Li Lin

    Verified customer

    Submitted Jan 07 2016

    1-10 of 23 Abreviews or Q&A

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