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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins
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RecombinantRabMAb

Recombinant Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (30)

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Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
  • Immunohistochemistry paraffin embedded sections - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
  • Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
  • Immunohistochemistry paraffin embedded sections - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
  • Immunohistochemistry paraffin embedded sections - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP913(2)Y] to E Cadherin (phospho S838 + S840)
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y]
    See all E Cadherin primary antibodies
  • Description

    Rabbit monoclonal [EP913(2)Y] to E Cadherin (phospho S838 + S840)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC: Human endometrium tissue, mouse and rat liver tissue; WB: Human, mouse, and rat brain.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP913(2)Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins
    • Developmental Biology
    • Organogenesis
    • Excretory system development
    • Kidney development

Associated products

  • Alternative Versions

    • Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] - BSA and Azide free (ab239883)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human E Cadherin protein (ab235682)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000. Predicted molecular weight: 97 kDa.

For unpurified use at 1/500000.

IHC-P (1)
1/80000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100.

Notes
WB
1/10000. Predicted molecular weight: 97 kDa.

For unpurified use at 1/500000.

IHC-P
1/80000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100.

  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
      E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
    • Tissue specificity

      Non-neural epithelial tissues.
    • Involvement in disease

      Defects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
      Defects in CDH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
      Defects in CDH1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    • Sequence similarities

      Contains 5 cadherin domains.
    • Post-translational
      modifications

      During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
    • Cellular localization

      Cell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
    • Target information above from: UniProt accession P12830 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 999 Human
      • Entrez Gene: 12550 Mouse
      • Entrez Gene: 83502 Rat
      • Omim: 192090 Human
      • SwissProt: P12830 Human
      • SwissProt: P09803 Mouse
      • SwissProt: Q9R0T4 Rat
      • Unigene: 461086 Human
      • Unigene: 35605 Mouse
      • Unigene: 1303 Rat
      see all
    • Alternative names

      • Arc 1 antibody
      • CADH1_HUMAN antibody
      • Cadherin 1 antibody
      • cadherin 1 type 1 E-cadherin antibody
      • Cadherin-1 antibody
      • Cadherin1 antibody
      • CAM 120/80 antibody
      • CD 324 antibody
      • CD324 antibody
      • CD324 antigen antibody
      • cdh1 antibody
      • CDHE antibody
      • E-Cad/CTF3 antibody
      • E-cadherin antibody
      • ECAD antibody
      • Epithelial cadherin antibody
      • epithelial calcium dependant adhesion protein antibody
      • LCAM antibody
      • Liver cell adhesion molecule antibody
      • UVO antibody
      • Uvomorulin antibody
      see all

    Images

    • Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
      Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
      All lanes : Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319) at 1/500000 dilution (Purified)

      Lane 1 : Human brain lysate, membrane treated with Alkaline Phosphatase for 1 hour
      Lane 2 : Human brain lysate
      Lane 3 : Rat brain lysate, membrane treated with Alkaline Phosphatase for 1 hour
      Lane 4 : Rat brain lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 97 kDa
      Observed band size: 145 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

    • Immunohistochemistry paraffin embedded sections - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
      Immunohistochemistry paraffin embedded sections - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling E Cadherin with Purified ab76319 at 1:80000 dilution (0.009 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
      Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
      Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319) at 1/10000 dilution (Purified) + Mouse brain lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 97 kDa
      Observed band size: 145 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

    • Immunohistochemistry paraffin embedded sections - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
      Immunohistochemistry paraffin embedded sections - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling E Cadherin with Purified ab76319 at 1:80000 dilution (0.009 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry paraffin embedded sections - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
      Immunohistochemistry paraffin embedded sections - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue sections labeling E Cadherin with Purified ab76319 at 1:80000 dilution (0.009 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)This image is courtesy of an anonymous Abreview

      Unpurified ab76319 staining E Cadherin in mouse skin (pilosebaceous untis) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with Tween-20 and blocked with 10% normal donkey serum + 1% serum for 40 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer, pH 6. Samples were incubated with primary antibody (1/400 in 1% BSA) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

      See Abreview

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (30)

    Publishing research using ab76319? Please let us know so that we can cite the reference in this datasheet.

    ab76319 has been referenced in 30 publications.

    • Ikonomou L  et al. The in vivo genetic program of murine primordial lung epithelial progenitors. Nat Commun 11:635 (2020). PubMed: 32005814
    • Zhang T  et al. MLPH Accelerates the Epithelial-Mesenchymal Transition in Prostate Cancer. Onco Targets Ther 13:701-708 (2020). PubMed: 32158222
    • Wang L MiR-141-3p overexpression suppresses the malignancy of osteosarcoma by targeting FUS to degrade LDHB. Biosci Rep 40:N/A (2020). PubMed: 32484203
    • Yuan L  et al. Resveratrol inhibits the invasion and metastasis of colon cancer through reversal of epithelial- mesenchymal transition via the AKT/GSK-3ß/Snail signaling pathway. Mol Med Rep 20:2783-2795 (2019). PubMed: 31524255
    • He C  et al. CircLMNB1 promotes colorectal cancer by regulating cell proliferation, apoptosis and epithelial-mesenchymal transition. Onco Targets Ther 12:6349-6359 (2019). PubMed: 31496737
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 40 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
    Sample
    Mouse Tissue sections (mouse skin, pilosebaceous units)
    Specification
    mouse skin, pilosebaceous units
    Permeabilization
    Yes - Tween-20
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 15 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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