Anti-E. coli antibody [9.88] - BSA and Azide free (ab269743)
Key features and details
- Mouse monoclonal [9.88] to E. coli - BSA and Azide free
- Suitable for: ELISA
- Isotype: IgM
Overview
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Product name
Anti-E. coli antibody [9.88] - BSA and Azide free
See all E. coli primary antibodies -
Description
Mouse monoclonal [9.88] to E. coli - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Predicted to work with: Escherichia coli
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Immunogen
Tissue, cells or virus corresponding to Escherichia coli E. coli. A crude sonicate of E. coli O157.
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General notes
ab269743 is a carrier free version of ab217910.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Purified IgM -
Clonality
Monoclonal -
Clone number
9.88 -
Isotype
IgM -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use a concentration of 1 - 5 µg/ml.
For coating use at 1-5 µg/ml. |
| Notes |
|---|
|
ELISA
Use a concentration of 1 - 5 µg/ml. For coating use at 1-5 µg/ml. |
Target
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Relevance
Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins. -
Alternative names
- Escherichia coli antibody
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab269743 has not yet been referenced specifically in any publications.
