Anti-E. coli antibody (ab13627)

Submit a review Q&A (11)References (8)

Key features and details

  • Goat polyclonal to E. coli
  • Suitable for: ICC/IF
  • Reacts with: Escherichia coli
  • Isotype: IgG

Overview

  • Product name

    Anti-E. coli antibody
    See all E. coli primary antibodies

  • Description

    Goat polyclonal to E. coli

  • Host species

    Goat

  • Specificity

    ab13627 reacts with many "O" and "K" antigenic serotypes of Escherichia coli.

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Escherichia coli

  • Immunogen

    Mixture of E. coli serotypes.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...

  • Purity

    IgG fraction

  • Purification notes

    Ab13627 consists of the purified IgG fraction prepared by methods developed by ViroStat to a purity of >95%.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab13627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
Notes
ICC/IF
Use at an assay dependent concentration.

Target

  • Relevance

    Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.

  • Alternative names

    • Escherichia coli antibody

References (8)

Publishing research using ab13627? Please let us know so that we can cite the reference in this datasheet.

ab13627 has been referenced in 8 publications.

  • Wu Z  et al. Pyroptosis engagement and bladder urothelial cell-derived exosomes recruit mast cells and induce barrier dysfunction of bladder urothelium after uropathogenic E. coli infection. Am J Physiol Cell Physiol 317:C544-C555 (2019). PubMed: 31241987
  • La Spina R  et al. Dark Field Microscopy-Based Biosensors for the Detection of E. coli in Environmental Water Samples. Sensors (Basel) 19:N/A (2019). PubMed: 31717745
  • Sarshar M  et al. A simple, fast and reliable scan-based technique as a novel approach to quantify intracellular bacteria. BMC Microbiol 19:252 (2019). PubMed: 31718545
  • Li X  et al. Compounds targeting YadC of uropathogenic Escherichia coli and its host receptor annexin A2 decrease bacterial colonization in bladder. EBioMedicine 50:23-33 (2019). PubMed: 31757778
  • Smyth D  et al. Interferon-gamma signals via an ERK1/2-ARF6 pathway to promote bacterial internalization by gut epithelia. Cell Microbiol 14:1257-70 (2012). PubMed: 22463716
  • Baccar H  et al. Surface plasmon resonance immunosensor for bacteria detection. Talanta 82:810-4 (2010). PubMed: 20602974
  • Mejri MB  et al. Impedance biosensing using phages for bacteria detection: generation of dual signals as the clue for in-chip assay confirmation. Biosens Bioelectron 26:1261-7 (2010). PubMed: 20673624
  • Berry RE  et al. Urothelial cultures support intracellular bacterial community formation by uropathogenic Escherichia coli. Infect Immun 77:2762-72 (2009). ICC/IF . PubMed: 19451249

Customer reviews and Q&As

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1-10 of 11 Abreviews or Q&A

Question

Comment a été purifié ab13627.

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Answer

Malheureusement la méthode de purification pour cet anticorps confidentielle, et donc nous ne pouvons pas partager les détails. Cependant, nous pouvons vous dire que la méthode utilise une précipitation du sulfate de sodium et donne une pureté équivalente à une méthode de précipitation de sulfate / méthode DEAE.

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Question

Inquiry: Hi, I obtained Anti-E. coli antibody (ab13627), lot n. GR97571-1. Could you please, tell me the concentration. Thanks

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Answer

Thank you for contacting us.

The concentration of ab13627 is approximately 4mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question

Inquiry: Hi, what is the concentration of the product ab13627/ lot no GR97571? Thanks.

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Answer

Thank you for your inquiry.

I am happy to confirm that the concentration of ab13627 is approximately 4 mg/ml.

I hope this information is helpful and wish you a good weekend.

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Question

Hi,

I am interested in the product Goat polyclonal antibody to e.coli. (ab13627). Can you tell me if there is any known cross reactivity with other species and what known serotypes this product reacts to? I am particularly keen for it to react to O157:H7. You mention that it will react to O157- does this automatically mean that it will react to all H proteins of the serotype of O157?

Kind Regards,

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Answer

Thank you for your enquiry.

Information in our files indicates this antibody has tested positive against the following serotypes:


O157:H7

O20

O125

O55

O111

K12

Unfortunately, I am sorry to confirm we have not specifically tested to see if it will recognize other H proteins of the O157 serotype. Information we have from the originators indicates they expects it react with many (if not all) O and K serotypes but the antibody has not been specifically tested against the H antigens.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

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Question

sorry, I'd like to know the binding serotypes for the antibody ab13627. In your answer you've reported for antibody ab20386.
Regards

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Answer

I apologize as I missed it.

Lab has confirmed the ab13627 is specific to O157, O20, O125, O55, O111 & K12 serotypes. However, they also advised that the immunogen was a mixture of several different serotypes and expect that the antibody would react with other O and K serotypes.

I hope this info will help.

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Question

Inquiry: I would like to know what is the concentration of the lot that has been sent to me ( Lot number GR67188 Product ab13627) Thank you very much for your help Thomas

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Answer

The concentration is 4mg/ml.
I hope this is helpful. Please contact us again if you have any further information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

ab13627 e coli antibody seems to be detecting something non specific - other bacteria?
We are staining cryostat sections of e coli in a population of macrophages. Then using a laser to remove the stained ecoli. Then we do PCR on the ecoli, and it seems to indicate there are other bacteria present
How specific is this antibody to e coli?

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Answer

Thank you for your telephone call yesterday afternoon. I am sorry to hear you have some concerns regarding the results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for IF. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

As discussed on the telephone, I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

I would appreciate if you could also provide an image of the staining which would help us to assess the results.

In answer to your questions regarding why the PCR is showing indication of presence of other bacteria, there are many factors to consider:

1. The IHC staining may need some optimization to ensure the staining of ecoli is accurate and correct, We can look at this with you and provide assistance.

2. The accuracy of the laser cutting, it may be cutting out other unstained cells.

3. The sterility of the samples, all the reagents and equipment are very important to ensure no contamination by other bacteria which may be picked up with the PCR.

4. How well the PCR is working, including negative controls? Could you confirm what other bacteria are being detected by the PCR? Do you have any further information or data?

Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.



Order Details
Antibody code:

Lot number:

Purchase order number
or preferably Abcam order number:


General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, low signal, non-specific satining etc.)

Sample (Species/Tissue/Cell Type/Cell Line etc.)


Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)


Antigen retrieval (Enzymatic method, Heat mediated technique etc.)


Permeabilization step


Blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method


Positive and negative controls used (please specify)


Optimization attempts (problem solving)
How many times have you tried the IHC?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No


What steps have you altered?


Additional Notes


We would appreciate if you are also able to provide and image which woudl help us to assess the results

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Question

We would like to conjugate this antibody with Dynabeads. Coudl you please tell us the conc of specific IgG or the dilution to be used in our assay?

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Answer

Thank you for your enquiry and your interest in our products.

I can confirm that ab13627 (anti-E. coli antibody) is not an affinity purified product so the IgG concentration is not been determined on a regular basis. We normally quantify and publish the concentration for affinity purified antibodies on the on-line product datasheets.

However, I can release that IgG concentration of our recent batch is 4.0 mg/ml. It is important, however, to emphasize that we currently do not have any experience in conjugating this particular antibody to Dynabeads.

If you need any further assistance in the future, please do not hesitate to contact me.

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Question

We would like to conjugate this antibody with Dynabeads. Coudl you please tell us the conc of specific IgG or the dilution to be used in our assay?

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Answer

Thank you for contacting us.

I can confirm that I have sent an email to my colleague in lab for the information you requested.

I will get back to you with a reply soon.

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Question


The discount is suitable for us.
Thank you for the attention.
Best regards,

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Answer

Thank you for your reply.

I have passed yourinformation and the discount information on to my colleagues on our customer services team, and they will be sending your proforma shortly.

Please let me know if you have any questions or if there is anything else that we can do for you. Have a wonderful day.

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1-10 of 11 Abreviews or Q&A

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