Overview

  • Product name
    Anti-E. coli antibody
    See all E. coli primary antibodies
  • Description
    Goat polyclonal to E. coli
  • Host species
    Goat
  • Specificity
    ab13627 reacts with many "O" and "K" antigenic serotypes of Escherichia coli.
  • Tested applications
    Suitable for: Conjugation, ICC/IFmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Mixture of E. coli serotypes.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Ab13627 consists of the purified IgG fraction prepared by methods developed by ViroStat to a purity of >95%.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • Relevance
    Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
  • Alternative names
    • Escherichia coli antibody

References

This product has been referenced in:
  • Baccar H  et al. Surface plasmon resonance immunosensor for bacteria detection. Talanta 82:810-4 (2010). Read more (PubMed: 20602974) »
  • Mejri MB  et al. Impedance biosensing using phages for bacteria detection: generation of dual signals as the clue for in-chip assay confirmation. Biosens Bioelectron 26:1261-7 (2010). Read more (PubMed: 20673624) »

See all 3 Publications for this product

Publishing research using ab13627? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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11 reviews or Q&A

10
Votes: Highest First

Comment a été purifié ab13627.

Malheureusement la méthode de purification pour cet anticorps confidentielle, et donc nous ne pouvons pas partager les détails. Cependant, nous pouvons vous dire que la méthode utilise une précipitation du sulfate de sodium et donne une pureté équivale...

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Inquiry: Hi, I obtained Anti-E. coli antibody (ab13627), lot n. GR97571-1. Could you please, tell me the concentration. Thanks

Thank you for contacting us.

The concentration of ab13627 is approximately 4mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit ...

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Inquiry: Hi, what is the concentration of the product ab13627/ lot no GR97571? Thanks.

Thank you for your inquiry.

I am happy to confirm that the concentration of ab13627 is approximately 4 mg/ml.

I hope this information is helpful and wish you a good weekend.

Hi,

I am interested in the product Goat polyclonal antibody to e.coli. (ab13627). Can you tell me if there is any known cross reactivity with other species and what known serotypes this product reacts to? I am particularly keen for it to rea...

Read More

Thank you for your enquiry.

Information in our files indicates this antibody has tested positive against the following serotypes:


O157:H7

O20

O125

O55

O111

K12

Unfo...

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sorry, I'd like to know the binding serotypes for the antibody ab13627. In your answer you've reported for antibody ab20386.
Regards

I apologize as I missed it.

Lab has confirmed the ab13627 is specific to O157, O20, O125, O55, O111 & K12 serotypes. However, they also advised that the immunogen was a mixture of several different serotypes and expect that the antibody wo...

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Inquiry: I would like to know what is the concentration of the lot that has been sent to me ( Lot number GR67188 Product ab13627) Thank you very much for your help Thomas

The concentration is 4mg/ml.
I hope this is helpful. Please contact us again if you have any further information.
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https://www.abcam.com/abreviews

ab13627 e coli antibody seems to be detecting something non specific - other bacteria?
We are staining cryostat sections of e coli in a population of macrophages. Then using a laser to remove the stained ecoli. Then we do PCR on the ecoli, and it ...

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Thank you for your telephone call yesterday afternoon. I am sorry to hear you have some concerns regarding the results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for IF....

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We would like to conjugate this antibody with Dynabeads. Coudl you please tell us the conc of specific IgG or the dilution to be used in our assay?

Thank you for your enquiry and your interest in our products.

I can confirm that ab13627 (anti-E. coli antibody) is not an affinity purified product so the IgG concentration is not been determined on a regular basis. We normally quantify and ...

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We would like to conjugate this antibody with Dynabeads. Coudl you please tell us the conc of specific IgG or the dilution to be used in our assay?

Thank you for contacting us.

I can confirm that I have sent an email to my colleague in lab for the information you requested.

I will get back to you with a reply soon.


The discount is suitable for us.
Thank you for the attention.
Best regards,

Thank you for your reply.

I have passed yourinformation and the discount information on to my colleagues on our customer services team, and they will be sending your proforma shortly.

Please let me know if you have any questions or ...

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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