Overview

  • Product name

  • Description

    Rabbit polyclonal to E. coli
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for many “O” and “K” antigenic serotypes of Escherichia coli. Antiserum is not absorbed and does cross-react with related Enterobacteriaceae. ab137967 will remove E. coli proteins from recombinant preparations.
  • Tested applications

    Suitable for: ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Escherichia coli
  • Immunogen

    Tissue, cells or virus corresponding to E. coli. Tissue/ cell preparation (Mixture of E. coli serotypes: K12, O111, O55, O125, O20, and O157).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Protein A chromatography. >95% pure.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance

    Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
  • Alternative names

    • Escherichia coli antibody

Images

  • Balb/C mice bearing s.c. CT26 flank tumours stained for E. coli (green) using ab137967 in IF. A donkey anti-rabbit Alexa Fluor 488-conjugated anti-Ig was used as the secondary antibody. Blue: DAPI.

References

This product has been referenced in:

  • Castro-Dopico T  et al. Anti-commensal IgG Drives Intestinal Inflammation and Type 17 Immunity in Ulcerative Colitis. Immunity 50:1099-1114.e10 (2019). Read more (PubMed: 30876876) »
  • Kromann S  et al. Treatment with high-dose antidepressants severely exacerbates the pathological outcome of experimental Escherichia coli infections in poultry. PLoS One 12:e0185914 (2017). Read more (PubMed: 29020098) »
See all 8 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer


Indeed we have no other specificity data for ab137967. I would therefore suggest one of the following antibodies:
Anti-E. coli antibody (ab25823) (The buffer contains trace amounts of citric acid and sodium phosphate. There are no preservatives or any other amine containing compounds and as such this material can be directly conjugated to enzymes, biotin etc. by most chemistries without the need to diafilter.)
Anti-E. coli antibody - Azide free (ab48416)

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Answer


The concentration of ab137967 ranges from 4-5 mg/ml.

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Answer

Thank you for contacting us.

The concentration of the product is between 4-5 mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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