Key features and details
- Rabbit polyclonal to E. coli
- Suitable for: ELISA, ICC/IF
- Reacts with: Escherichia coli
- Isotype: IgG
Product nameAnti-E. coli antibody
See all E. coli primary antibodies
DescriptionRabbit polyclonal to E. coli
SpecificityThis antibody is specific for many “O” and “K” antigenic serotypes of Escherichia coli. Antiserum is not absorbed and does cross-react with related Enterobacteriaceae. ab137967 will remove E. coli proteins from recombinant preparations.
Tested applicationsSuitable for: ELISA, ICC/IFmore details
Species reactivityReacts with: Escherichia coli
Tissue, cells or virus corresponding to E. coli. Tissue/ cell preparation (Mixture of E. coli serotypes: K12, O111, O55, O125, O20, and O157).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesProtein A chromatography. >95% pure.
Our Abpromise guarantee covers the use of ab137967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
RelevanceEscherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
- Escherichia coli antibody
ab137967 has been referenced in 9 publications.
- Castro-Dopico T et al. Anti-commensal IgG Drives Intestinal Inflammation and Type 17 Immunity in Ulcerative Colitis. Immunity 50:1099-1114.e10 (2019). PubMed: 30876876
- Jain N & Vogel V Spatial confinement downsizes the inflammatory response of macrophages. Nat Mater 17:1134-1144 (2018). PubMed: 30349032
- Murphy C et al. Intratumoural production of TNFa by bacteria mediates cancer therapy. PLoS One 12:e0180034 (2017). IF ; Mouse . PubMed: 28662099
- Kromann S et al. Treatment with high-dose antidepressants severely exacerbates the pathological outcome of experimental Escherichia coli infections in poultry. PLoS One 12:e0185914 (2017). PubMed: 29020098
- Zhang YS et al. Hybrid Microscopy: Enabling Inexpensive High-Performance Imaging through Combined Physical and Optical Magnifications. Sci Rep 6:22691 (2016). PubMed: 26975883
- Russo BC et al. Intermediate filaments enable pathogen docking to trigger type 3 effector translocation. Nat Microbiol 1:16025 (2016). PubMed: 27572444
- Reeves AZ et al. Engineering Escherichia coli into a protein delivery system for mammalian cells. ACS Synth Biol 4:644-54 (2015). IF . PubMed: 25853840
- Hoppe S et al. Identification of antigenic proteins of the nosocomial pathogen Klebsiella pneumoniae. PLoS One 9:e110703 (2014). PubMed: 25333280
- Rossez Y et al. Escherichia coli common pilus (ECP) targets arabinosyl residues in plant cell walls to mediate adhesion to fresh produce plants. J Biol Chem 289:34349-65 (2014). PubMed: 25320086