• Product name
  • Description
    Goat polyclonal to E. coli
  • Host species
  • Specificity
    ab25823 recognises all E. coli species tested. The following species of E. coli were used in the antigen prep: Detergent lysed E. coli from K12, TOP 10F, XS127/P3, JM109,HB101, and DH5a and BL21. We have also extensively tested K-12 Since there is 95% homology between the strains, all species of E.coli should react with this antibody.
  • Tested applications
    Suitable for: ICC/IF, Electron Microscopy, WB, ELISA, Dot blotmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Tissue, cells or virus corresponding to E. coli. Tissue/ cell preparation (Escherichia coli ): Detergent lysed E. coli (cocktail blend of strains of E. coli including the most commonly used DH5a and BL21).


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituents: 0.595% HEPES, 0.87% Sodium chloride

    The buffer contains trace amounts of citric acid and sodium phosphate. There are no preservatives or any other amine containing compounds and as such this material can be directly conjugated to enzymes, biotin etc. by most chemistries without the need to diafilter.
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab25823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 22427638
Electron Microscopy Use at an assay dependent concentration. PubMed: 24253282
WB Use a concentration of 0.5 - 2 µg/ml. Note: Reacts with more than 50 proteins by Western Blot.
ELISA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.


  • Relevance
    Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
  • Alternative names
    • Escherichia coli antibody


This product has been referenced in:
  • Farka Z  et al. Rapid detection of microorganisms based on active and passive modes of QCM. Sensors (Basel) 15:79-92 (2014). Read more (PubMed: 25545267) »
  • Nayak M  et al. Integrated sorting, concentration and real time PCR based detection system for sensitive detection of microorganisms. Sci Rep 3:3266 (2013). Electron Microscopy ; Escherichia coli . Read more (PubMed: 24253282) »

See all 6 Publications for this product

Customer reviews and Q&As

Escherichia coli Cell (K12 bacterial solution)
K12 bacterial solution
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1%

Abcam user community

Verified customer

Submitted May 13 2014

The Goat polyclonal anti-E. coli conjugated to HRP is ab69185. The link to the datasheet is https://www.abcam.com/ab69185.

Thank you for contacting us.

We only supply this antibody in 100 ug sizes however, if you were to purchase 5 or more vials of this product, it would be possible for me to give you a discount.

If you could let me know how many vial...

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Thank you for contacting us.

The K12 strain was one of the strains used to generate the antibody.

This antibody was actually raised against a blend of lysates from both BL21 and DH5a and as a result will detect the majority of...

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Thank you for contacting Abcam.

The SDS for ab25823 has been created and will be posted on the website by the "Datasheet PDF" tab within the next 48 hours (https://www.abcam.com/e-coli-antibody-ab25823.html).

I hope this inf...

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Thank you for your email.

I understand your concerns that antibodies should form immune complex with compatible antigens irrespective of their location however unfortunately this is not the case. Antibodies behaves differently in each applicat...

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;


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I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application us...

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Thank you for contacting us.

I have heard back from the lab with the following answers:

1. ab25823 can be used for Western blot in the concentration range of 0.5 to 2ug/mL.

2. We have not tested the O157 strain for cross...

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Merci de nous avoir contactés.
Comme expliqué par téléphone j'ai mis en pièce jointe un questionnaire qui nous permettra de regarder plus en détails votre protocole.
Les liens vers les protocoles Abcam pour la cytométrie en flux sont:
> htt...

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